Strickland R W, Wahl L M, Finbloom D S
J Immunol. 1986 Sep 1;137(5):1577-80.
Human peripheral blood monocytes possess receptors on their surface for human interferon-gamma (IFN-gamma). Inasmuch as the biologic effects of IFN-gamma are thought to be mediated through binding of the molecule to this receptor, modulation of receptor number may be a means for enhancing the effect of IFN-gamma. We examined this possibility by treating human monocytes in culture with dexamethasone. At a dexamethasone concentration of 200 nM, there was a twofold increase in the number of 125I-recombinant IFN-gamma molecules bound to the cell. An effect was noted at concentrations of dexamethasone greater than 50 nM, and was observed as early as after 18 hr of treatment. The observed effect was the result of both a 50% decrease in receptor number in untreated cells and an increase to pretreatment levels or greater in cells incubated with dexamethasone. These results suggest that dexamethasone may modulate the effects of IFN-gamma on monocytes by changes in receptor number.
人类外周血单核细胞表面存在人类γ干扰素（IFN-γ）的受体。由于IFN-γ的生物学效应被认为是通过该分子与受体结合来介导的，因此调节受体数量可能是增强IFN-γ效应的一种方式。我们通过用地塞米松处理培养的人类单核细胞来研究这种可能性。在地塞米松浓度为200 nM时，与细胞结合的125I重组IFN-γ分子数量增加了两倍。在地塞米松浓度大于50 nM时就观察到了这种效应，并且早在处理18小时后就出现了。观察到的效应是由于未处理细胞中受体数量减少50%，以及在用糖皮质激素孵育的细胞中受体数量增加到预处理水平或更高水平所致。这些结果表明，地塞米松可能通过改变受体数量来调节IFN-γ对单核细胞的作用。
