• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

Preparation of Antisense Oligonucleotides to Inhibit miRNA Function.

作者信息

Li Chengjian, Zamore Phillip D

出版信息

Cold Spring Harb Protoc. 2018 Feb 1;2018(2):2018/2/pdb.prot097527. doi: 10.1101/pdb.prot097527.

DOI:10.1101/pdb.prot097527
PMID:29438060
Abstract

This protocol is used to design antisense oligonucleotides (ASOs) for specific inhibition of miRNA function in cultured cells. The incorporation of 2'--methyl-modifications in ASOs enhances their potency and resistance to degradation, whereas 3'-terminal cholesterol-conjugation facilitates delivery of ASOs into cells.

摘要

相似文献

1
Preparation of Antisense Oligonucleotides to Inhibit miRNA Function.
Cold Spring Harb Protoc. 2018 Feb 1;2018(2):2018/2/pdb.prot097527. doi: 10.1101/pdb.prot097527.
2
Inhibiting miRNA Function by Antisense Oligonucleotides in Cultured Mammalian Cells.
Cold Spring Harb Protoc. 2018 Feb 1;2018(2):2018/2/pdb.prot097535. doi: 10.1101/pdb.prot097535.
3
Inhibiting miRNA Function by Antisense Oligonucleotides in S2 Cells.
Cold Spring Harb Protoc. 2018 Feb 1;2018(2):2018/2/pdb.prot097543. doi: 10.1101/pdb.prot097543.
4
Design and delivery of antisense oligonucleotides to block microRNA function in cultured Drosophila and human cells.设计并递送反义寡核苷酸以阻断培养的果蝇和人类细胞中的微小RNA功能。
Nat Protoc. 2008;3(10):1537-49. doi: 10.1038/nprot.2008.145.
5
Non-nucleotide Modification of Anti-miRNA Oligonucleotides.抗微小RNA寡核苷酸的非核苷酸修饰
Methods Mol Biol. 2017;1517:51-69. doi: 10.1007/978-1-4939-6563-2_3.
6
Chemical modification and design of anti-miRNA oligonucleotides.反 miRNA 寡核苷酸的化学修饰与设计。
Gene Ther. 2011 Dec;18(12):1111-20. doi: 10.1038/gt.2011.100. Epub 2011 Jul 14.
7
Hsp90 protein interacts with phosphorothioate oligonucleotides containing hydrophobic 2'-modifications and enhances antisense activity.热休克蛋白90（Hsp90）与含有疏水性2'-修饰的硫代磷酸酯寡核苷酸相互作用，并增强反义活性。
Nucleic Acids Res. 2016 May 5;44(8):3892-907. doi: 10.1093/nar/gkw144. Epub 2016 Mar 3.
8
Improved targeting of miRNA with antisense oligonucleotides.利用反义寡核苷酸改善对微小RNA的靶向作用。
Nucleic Acids Res. 2006 May 11;34(8):2294-304. doi: 10.1093/nar/gkl183. Print 2006.
9
(S)-5'-C-Aminopropyl-2'-O-methyl nucleosides enhance antisense activity in cultured cells and binding affinity to complementary single-stranded RNA.(S)-5'-C-氨丙基-2'-O-甲基核苷增强了培养细胞中的反义活性和与互补单链 RNA 的结合亲和力。
Bioorg Med Chem. 2021 Jan 15;30:115925. doi: 10.1016/j.bmc.2020.115925. Epub 2020 Dec 7.
10
Potent inhibition of microRNA in vivo without degradation.在体内对微小RNA进行有效抑制而不发生降解。
Nucleic Acids Res. 2009 Jan;37(1):70-7. doi: 10.1093/nar/gkn904. Epub 2008 Nov 16.

引用本文的文献

1
CRISPR-Cas knockout of miR21 reduces glioma growth.CRISPR-Cas介导的miR21基因敲除可降低胶质瘤的生长。
Mol Ther Oncolytics. 2022 Apr 6;25:121-136. doi: 10.1016/j.omto.2022.04.001. eCollection 2022 Jun 16.
2
Interplay of SOX transcription factors and microRNAs in the brain under physiological and pathological conditions.生理和病理条件下大脑中SOX转录因子与微小RNA的相互作用
Neural Regen Res. 2022 Nov;17(11):2325-2334. doi: 10.4103/1673-5374.338990.
3
The role of MiRNA-21 in gliomas: Hope for a novel therapeutic intervention?
微小RNA-21在神经胶质瘤中的作用：新型治疗干预的希望？
Toxicol Rep. 2020 Nov 6;7:1514-1530. doi: 10.1016/j.toxrep.2020.11.001. eCollection 2020.