Branca A A
J Interferon Res. 1986 Jun;6(3):305-11. doi: 10.1089/jir.1986.6.305.
Membrane fractions were prepared from fresh frozen bovine lung tissue and human placenta and used for receptor binding studies with recombinant DNA produced human-alpha-interferon (IFN-alpha). A single class of high-affinity binding sites was determined for bovine lung (Ka = 1.5 X 10(10) M) and human placenta (Ka = 3.7 X 10(9) M) membranes, respectively. These values for the affinity of IFN binding are comparable within the range of observed error to that observed for the binding of human IFN-alpha 2 to cultured Daudi cells (Ka = 2.5 X 10(10) M). The type I IFN receptor content of bovine lung and human placenta membranes was 1.3 and 2.5 fmoles/mg wet weight, respectively. In addition, alterations in specific and nonspecific binding were observed with the bovine lung membrane incubations in the presence of calcium. Increases in specific binding of three- to fourfold were observed in the presence of 1 mM calcium chloride.
从新鲜冷冻的牛肺组织和人胎盘中制备膜组分，并用于与重组DNA产生的人α-干扰素（IFN-α）进行受体结合研究。分别测定了牛肺（Ka = 1.5×10¹⁰ M）和人胎盘（Ka = 3.7×10⁹ M）膜的一类高亲和力结合位点。这些IFN结合亲和力的值在观察误差范围内与观察到的人IFN-α 2与培养的Daudi细胞结合的值（Ka = 2.5×10¹⁰ M）相当。牛肺和人胎盘膜的I型IFN受体含量分别为1.3和2.5 fmol/mg湿重。此外，在存在钙的情况下进行牛肺膜孵育时，观察到特异性和非特异性结合的变化。在存在1 mM氯化钙的情况下，观察到特异性结合增加了三到四倍。
