State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China; College of Life Science, Hunan Normal University, Changsha 410006, China.
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
J Insect Physiol. 2018 May-Jun;107:14-22. doi: 10.1016/j.jinsphys.2018.02.005. Epub 2018 Feb 10.
The highly specialized olfactory receptor neurons (ORNs) on the antennae of male moths can recognize blends of several pheromone components. In previous studies, a total of six candidate pheromone receptor (PR) genes were cloned and functionally characterized in the diamondback moth, Plutella xylostella. In the present work, we report on three novel candidate pheromone receptor genes: PxylOR8, PxylOR41, and PxylOR45 in the same species. Gene expression analysis revealed that PxylOR8 is specifically expressed in female adult antennae, while PxylOR41 and PxylOR45 are expressed in antennae in both sexes, but with a male bias. In situ hybridization revealed that PxylOR8, PxylOR41 and PxylOR45 are localized in long trichoid sensilla. Functional analyses on the three pheromone receptor genes were then performed using the heterologous expression system of Xenopus oocytes. PxylOR41 was tuned to two minor pheromone components Z9-14:Ac, Z9-14:OH, and their analog Z9-14:Ald. PxylOR8 and PxylOR45 did not respond to any tested pheromone components and analogs. These results may contribute to clarifying how pheromone detection works in P. xylostella.
触角上高度特化的嗅觉受体神经元 (ORNs) 能够识别几种信息素成分的混合物。在之前的研究中，总共克隆并功能表征了小菜蛾中的 6 个候选信息素受体 (PR) 基因。在本工作中，我们在同一物种中报告了三个新的候选信息素受体基因：PxylOR8、PxylOR41 和 PxylOR45。基因表达分析显示 PxylOR8 特异性表达在雌性成虫触角中，而 PxylOR41 和 PxylOR45 在两性触角中均有表达，但雄性偏多。原位杂交显示 PxylOR8、PxylOR41 和 PxylOR45 定位于长刚毛感觉器。然后使用非洲爪蟾卵母细胞的异源表达系统对这三个信息素受体基因进行了功能分析。PxylOR41 对两种次要信息素成分 Z9-14:Ac、Z9-14:OH 及其类似物 Z9-14:Ald 有响应。PxylOR8 和 PxylOR45 对任何测试的信息素成分及其类似物均无反应。这些结果可能有助于阐明小菜蛾中信息素检测的工作原理。
