He Xiaoli, Cai Yajie, Zhu Jinglei, Zhang Mengdi, Zhang Yadong, Ge Yang, Zhu Zengrong, Zhou Wenwu, Wang Guirong, Gao Yulin
Institute of Insect Sciences, Key Laboratory of Biology of Crop Pathogens and Insects of Zhejiang Province, Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Ministry of Agriculture, State Key Laboratory of Rice Biology, Hangzhou, China.
State Key Laboratory for Biology of Plant Disease and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China.
Front Physiol. 2021 Jan 25;11:618983. doi: 10.3389/fphys.2020.618983. eCollection 2020.
Pheromones are a kind of signal produced by an animal that evoke innate responses in conspecifics. In moth, pheromone components can be detected by specialized olfactory receptor neurons (OSNs) housed in long sensilla trichoids on the male antennae. The pheromone receptors (PRs) located in the dendrite membrane of OSNs are responsible for pheromone sensing in most Lepidopteran insects. The potato tuber moth is a destructive pest of Solanaceae crops. Although sex attractant is widely used in fields to monitor the population of , no study has been reported on the mechanism the male moth of uses to recognize sex pheromone components. In the present study, we cloned two pheromone receptor genes and in . The transcripts of them were highly accumulated in the antennae of male adults. Functional analysis using the heterologous expression system of oocyte demonstrated that these two PR proteins both responded to (, )-4,7-13: OAc and (, , )-4,7,10-13: OAc, the key sex pheromone components of , whilst they responded differentially to these two ligands. Our findings for the first time characterized the function of pheromone receptors in gelechiid moth and could promote the olfactory based pest management of in the field.
信息素是动物产生的一种信号，可在同种个体中引发先天反应。在蛾类中，信息素成分可由位于雄蛾触角上长刚毛状感器中的特殊嗅觉受体神经元（OSNs）检测到。位于OSNs树突膜上的信息素受体（PRs）负责大多数鳞翅目昆虫的信息素感知。马铃薯块茎蛾是茄科作物的一种毁灭性害虫。尽管性引诱剂在田间被广泛用于监测其种群数量，但尚未有关于马铃薯块茎蛾雄蛾识别性信息素成分机制的研究报道。在本研究中，我们克隆了马铃薯块茎蛾中的两个信息素受体基因。它们的转录本在雄性成虫触角中高度积累。使用卵母细胞异源表达系统进行的功能分析表明，这两种PR蛋白均对马铃薯块茎蛾的关键性信息素成分（顺,反）-4,7-13:OAc和（顺,顺,反）-4,7,10-13:OAc有反应，不过它们对这两种配体的反应存在差异。我们的研究结果首次明确了麦蛾科蛾类中信息素受体的功能，并有助于促进基于嗅觉的马铃薯块茎蛾田间害虫治理。
