School of Biochemical Engineering, Indian Institute of Technology (Banaras Hindu University), Varanasi 221005, India.
Department of Orthopedics, Institute of Medical Sciences, Banaras Hindu University, Varanasi 221005, India.
Int J Biol Macromol. 2018 Jun;112:909-920. doi: 10.1016/j.ijbiomac.2018.02.049. Epub 2018 Feb 10.
Poor regenerative potential of cartilage tissue due to the avascular nature and lack of supplementation of reparative cells impose an important challenge in recent medical practice towards development of artificial extracellular matrix with enhanced neo-cartilage tissue regeneration potential. Chitosan (CH), poly (l-lactide) (PLLA), and pectin (PC) compositions were tailored to generate polyelectrolyte complex based porous scaffolds using freeze drying method and crosslinked by 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC), N-hydroxysuccinimide (NHS) solution containing chondroitin sulfate (CS) to mimic the composition as well as architecture of the cartilage extracellular matrix (ECM). The physical, chemical, thermal, and mechanical behaviors of developed scaffolds were done. The scaffolds were porous with homogeneous pore structure with pore size 49-170μm and porosities in the range of 79 to 84%. Fourier transform infrared study confirmed the presence of polymers (CH, PLLA and PC) within the scaffolds. The crystallinity of the scaffold was examined by the X-ray diffraction studies. Furthermore, scaffold shows suitable swelling property, moderate biodegradation and hemocompatibility in nature and possess suitable mechanical strength for cartilage tissue regeneration. MTT assay, GAG content, and attachment of chondrocyte confirmed the regenerative potential of the cell seeded scaffold. The histopathological analysis defines the suitability of scaffold for cartilage tissue regeneration.
由于软骨组织的血管稀少和缺乏修复细胞的补充，其再生潜力较差，这对当前的医学实践提出了重要挑战，即开发具有增强的新软骨组织再生潜力的人工细胞外基质。壳聚糖 (CH)、聚 (L-丙交酯) (PLLA) 和果胶 (PC) 组成通过冷冻干燥法进行定制，以生成基于聚电解质复合物的多孔支架，并通过 1-乙基-3-(3-二甲基氨基丙基)碳二亚胺 (EDC)、N-羟基琥珀酰亚胺 (NHS) 溶液交联，其中含有硫酸软骨素 (CS)，以模拟软骨细胞外基质 (ECM) 的组成和结构。对开发的支架进行了物理、化学、热和机械性能的测试。支架具有均匀的孔结构，孔径为 49-170μm，孔隙率在 79%至 84%之间。傅里叶变换红外研究证实了聚合物 (CH、PLLA 和 PC) 存在于支架中。通过 X 射线衍射研究检查了支架的结晶度。此外，支架具有适当的溶胀性能、适度的生物降解性和天然的血液相容性，具有适合软骨组织再生的机械强度。MTT 测定、GAG 含量和软骨细胞的附着证实了细胞接种支架的再生潜力。组织病理学分析定义了支架用于软骨组织再生的适宜性。
