Huang T R, Wang G C, Zhang H M, Peng B
Department of Urology, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai, China.
Andrologia. 2018 Feb 14. doi: 10.1111/and.12974.
Prostate cancer (PCa) is one of the most common male malignancies in the world. It was aimed to investigate differential expression of inflammatory and related factors in benign prostatic hyperplasia (BPH), prostate cancer (PCa), histological prostatitis (HP) and explore the role of Inducible nitric oxide synthase (iNOS), (VEGF) Vascular endothelial growth factor, androgen receptor (AR) and IL-2, IL-8 and TNF-α in the occurrence and development of prostate cancer. RT-PCR was used to detect the mRNA expression level of iNOS, VEGF, AR and IL-2, IL-8 and TNF-α in BPH, PCa and BPH+HP. Western blotting and immunohistochemical staining were used to detect the protein levels of various proteins in three diseases. The results showed the mRNA and protein levels of iNOS, VEGF and IL-2, IL-8 and TNF-α were significantly increased in PCa and BPH+HP groups compared with BPH group (p < .05), while the AR was significantly lower than those in PCa and BPH+HP groups (p < .05). There was no significant difference in the mRNA and protein levels of iNOS, VEGF, AR and IL-2, IL-8 and TNF-α between PCa and BPH+HP groups (p > .05). iNOS, VEGF, AR and IL-2, IL-8 and TNF-α are involved in the malignant transformation of prostate tissue and play an important role in the development and progression of Prostate cancer (PCa).
前列腺癌（PCa）是全球最常见的男性恶性肿瘤之一。旨在研究良性前列腺增生（BPH）、前列腺癌（PCa）、组织学前列腺炎（HP）中炎症及相关因子的差异表达，并探讨诱导型一氧化氮合酶（iNOS）、血管内皮生长因子（VEGF）、雄激素受体（AR）以及白细胞介素-2（IL-2）、白细胞介素-8（IL-8）和肿瘤坏死因子-α（TNF-α）在前列腺癌发生发展中的作用。采用逆转录聚合酶链反应（RT-PCR）检测BPH、PCa和BPH+HP中iNOS、VEGF、AR以及IL-2、IL-8和TNF-α的mRNA表达水平。采用蛋白质印迹法和免疫组织化学染色检测三种疾病中各种蛋白质的水平。结果显示，与BPH组相比，PCa组和BPH+HP组中iNOS、VEGF以及IL-2、IL-8和TNF-α的mRNA和蛋白质水平显著升高（p<0.05），而AR水平显著低于PCa组和BPH+HP组（p<0.05）。PCa组和BPH+HP组之间iNOS、VEGF、AR以及IL-2、IL-8和TNF-α的mRNA和蛋白质水平无显著差异（p>0.05）。iNOS、VEGF、AR以及IL-2、IL-8和TNF-α参与前列腺组织的恶性转化，在前列腺癌（PCa）的发生发展中起重要作用。
