Gao L N, Yuan H Y, Xu E Y, Liu J T
School of Forensic Medicine, China Medical University, Shenyang 110013, China.
Fa Yi Xue Za Zhi. 2017 Dec;33(6):619-621. doi: 10.3969/j.issn.1004-5619.2017.06.011. Epub 2017 Dec 25.
To establish a gas chromatographic-mass spectrometric （GC-MS） analysis method for quantifying 1-methylhydantoin concentration in whole blood. To provide technical support to forensic identification related cases of 1-methylhydantoin.
As an internal standard, 500 ng SKF was added to 0.5 mL blood sample, and then 2 mL 0.01 mol/L dilute hydrochloric acid and 0.5 g ammonium carbonate were added in order to buffer the pH value to 9, and following 2 mL ethyl acetate. The organic solvent layer was obtained after centrifuge and then analysed by GC-MS after drying.
Good linear relationship of 1-methylhydantoin in blood was obtained in the range of 0.5-50 ng/mL. The equation of linear regression was =0.015 51 +0.007 26（²=0.999 7） with 0.1 ng/mL detection limit, and the recovery was 93.02%-108.12%. The intra-day and inter-day precision were less than 6.07% and 13.37%, respectively.
The results gotten by this method is accurate and reproducible, which can be used for the determination of 1-methylhydantoin concentration in blood samples.
