[气相色谱-质谱联用测定人血中丙泊酚的含量]
[Determination of propofol in human blood by GC-MS].
作者信息
Sun Ying-Ying, Xiang Ping, Liu Wei, Bu Jun, Shen Min
机构信息
Shanghai Key Laboratory of Forensic Medicine, Institute of Forensic Science, Ministry of Justice, PR China, Shanghai 200063, China.
出版信息
Fa Yi Xue Za Zhi. 2011 Dec;27(6):430-3.
OBJECTIVE
To establish a gas chromatography-mass spectrometry (GC-MS) method for determination of propofol in human blood.
METHODS
Propofol and 2-(tert-Butyl)-4,6-dimethylphenol (internal standard) were isolated from human blood samples with liquid-liquid ether extraction. The organic layer was collected after centrifugation and dried using the water bath. The extracted residue was analyzed by GC-MS.
RESULTS
Propofol and the internal standard showed a good separation with a good linear concentration ranged from 0.02 to 10 microg/mL in blood. The linear function was y = 0.313 6 x-0.006 8 with the correlation coefficient of 0.9997. The precision of intra-day and inter-day were less than 4.8% and the lower limit of detection of propofol was 0.005 microg/mL. Propofol concentration of blood was 0.14 microg/mL using this method in the practice work.
CONCLUSION
The GC-MS method is rapid, sensitive, reliable and suitable for qualitative and quantitative analysis propofol of blood in forensic toxicological analysis and clinical drug monitoring.
目的
建立气相色谱-质谱联用（GC-MS）法测定人血液中丙泊酚的含量。
方法
采用液-液乙醚萃取法从人血样中分离丙泊酚和2-（叔丁基）-4,6-二甲基苯酚（内标）。离心后收集有机层，用水浴干燥。萃取残渣用GC-MS分析。
结果
丙泊酚和内标分离良好，在血液中的线性浓度范围为0.02至10μg/mL，线性方程为y = 0.313 6x - 0.006 8，相关系数为0.9997。日内和日间精密度均小于4.8%，丙泊酚的检测下限为0.005μg/mL。实际工作中采用该方法测得血液中丙泊酚浓度为0.14μg/mL。
结论
GC-MS法快速、灵敏、可靠，适用于法医毒理学分析和临床药物监测中血液中丙泊酚的定性和定量分析。
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