Yuan Jinjin, Liu Zongwen, Song Rui
Pharmazie. 2017 Jul 3;72(7):402-407. doi: 10.1691/ph.2017.7449.
Antisense lncRNAs play a key role in the progression of multiple cancers. Thus, it is important to elucidate the function and mechanism of antisense lncRNAs, which may play a role in the treatment of epithelial ovarian cancer (EOC). In the current study, for the first time, we showed that the level of As-SLC7A11 was markedly reduced in EOC cancer tissues and cell lines compared with those of normal control. Further study showed that silencing of As-SLC7A11 could enhance ovarian cancer cell migration. In comparison, overexpression of As-SLC7A11 markedly induced ovarian cancer cell apoptosis. These data demonstrate the tumor suppressor role of As-SLC7A11 in ovarian cancer malignancies. We also demonstrated that overexpression of As-SLC7A11 could significantly suppress the expression of SLC7A11, indicating a negative correlation between As-SLC7A11 and SLC7A11 in ovarian cancer cells. In summary, we first showed that reduction of As-SLC7A11 level prompted ovarian cancer cell migration mainly by suppressing the expression of SLC7A11.
反义长链非编码RNA（lncRNAs）在多种癌症进展中起关键作用。因此，阐明反义lncRNAs的功能和机制很重要，其可能在上皮性卵巢癌（EOC）治疗中发挥作用。在本研究中，我们首次发现，与正常对照相比，EOC癌组织和细胞系中As-SLC7A11水平显著降低。进一步研究表明，沉默As-SLC7A11可增强卵巢癌细胞迁移。相比之下，过表达As-SLC7A11显著诱导卵巢癌细胞凋亡。这些数据证明了As-SLC7A11在卵巢癌恶性肿瘤中的肿瘤抑制作用。我们还证明，过表达As-SLC7A11可显著抑制SLC7A11的表达，表明卵巢癌细胞中As-SLC7A11与SLC7A11呈负相关。总之，我们首次表明，As-SLC7A11水平降低主要通过抑制SLC7A11的表达促使卵巢癌细胞迁移。
