Department of Laboratory Medicine, Affiliated Hospital of Nantong University, Nantong, Jiangsu, China.
Medical School of Nantong University, Nantong University, Nantong, Jiangsu, China.
J Cell Mol Med. 2024 Sep;28(17):e70061. doi: 10.1111/jcmm.70061.
Long non-coding RNAs (lncRNAs) play an important role in the progression of gastric cancer (GC), but its specific regulatory mechanism remains to be further studied. We previously identified that lncRNA B3GALT5-AS1 was upregulated in GC serum. Here, we investigated the functions and molecular mechanisms of B3GALT5-AS1 in GC tumorigenesis. qRT-PCR was used to detect B3GALT5-AS1 expression in GC. EdU, CCK-8, and colony assays were utilized to assess the proliferation ability of B3GAL5-AS1, and transwell, tube formation assay were used to assess the invasion and metastasis ability. Mechanically, FISH and nuclear plasmolysis PCR identified the subcellular localization of B3GALT5-AS1. RIP and CHIP assays were used to analyse the regulation of B3GALT5-AS1 and B3GALT5. We observed that B3GALT5-AS1 was highly expressed in GC, and silencing B3GALT5-AS1 could inhibit the proliferation, invasion, and migratory capacities of GC. Additionally, B3GALT5-AS1 was bound to WDR5 and modulated the expression of B3GALT5 via regulating the ZEB1/β-catenin pathway. High-expressed B3AGLT5-AS1 promoted GC tumorigenesis and regulated B3GALT5 expression via recruiting WDR5. Our study is expected to provide a new idea for clinical diagnosis and treatment.
长链非编码 RNA（lncRNAs）在胃癌（GC）的进展中发挥着重要作用，但具体的调控机制仍有待进一步研究。我们之前发现 lncRNA B3GALT5-AS1 在 GC 血清中上调。在这里，我们研究了 B3GALT5-AS1 在 GC 肿瘤发生中的功能和分子机制。qRT-PCR 用于检测 GC 中 B3GALT5-AS1 的表达。EdU、CCK-8 和集落测定用于评估 B3GAL5-AS1 的增殖能力，Transwell 和管形成测定用于评估侵袭和转移能力。从机制上讲，FISH 和核质分离 PCR 确定了 B3GALT5-AS1 的亚细胞定位。RIP 和 CHIP 测定用于分析 B3GALT5-AS1 和 B3GALT5 的调节。我们观察到 B3GALT5-AS1 在 GC 中高度表达，沉默 B3GALT5-AS1 可以抑制 GC 的增殖、侵袭和迁移能力。此外，B3GALT5-AS1 与 WDR5 结合，并通过调节 ZEB1/β-catenin 通路调节 B3GALT5 的表达。高表达的 B3AGLT5-AS1 通过招募 WDR5 促进 GC 肿瘤发生并调节 B3GALT5 的表达。我们的研究有望为临床诊断和治疗提供新的思路。
