Chilton B S, Gettys K L, Newman M G, McAllister C J, Cowart K T
Steroids. 1985 Oct-Nov;46(4-5):915-27. doi: 10.1016/0039-128x(85)90039-x.
Uteri and cervices were obtained from estrous rabbits (controls) and from rabbits 24 h or 7 days after a single intramuscular injection of medroxyprogesterone acetate (MPA; 2.14 mg/kg). Estrogen and progesterone receptor concentrations were measured by Scatchard analysis, cell-free DNA synthesis was measured by (3H)-TTP incorporation, and tissue sections were examined histologically. The uterine endometrium underwent marked changes in histology, including extensive infoldings of the mucosal surface, glands were continuous into crypts and secretory epithelial cells were noted. In addition, total estrogen receptor content and DNA synthesis were decreased. In contrast, there was no significant change in the histology of the endocervical epithelial-stromal complex, and total estrogen receptor remained constant. However, DNA synthesis in the endocervix was decreased. Thus we conclude that: DNA synthesis is not linked to changes in estrogen receptor in the endocervix; and differential effects of progestogen on the estrogen receptor system occur coincident with different morphological responses within two target tissues from the same animal.
子宫和子宫颈取自处于发情期的兔子（对照组）以及单次肌肉注射醋酸甲羟孕酮（MPA；2.14毫克/千克）24小时或7天后的兔子。通过Scatchard分析测定雌激素和孕激素受体浓度，通过（3H）-TTP掺入法测定无细胞DNA合成，并对组织切片进行组织学检查。子宫内膜在组织学上发生了显著变化，包括黏膜表面广泛折叠，腺体延伸至隐窝并可见分泌上皮细胞。此外，雌激素受体总量和DNA合成减少。相比之下，子宫颈上皮-基质复合体的组织学没有显著变化，雌激素受体总量保持恒定。然而，子宫颈中的DNA合成减少。因此我们得出结论：DNA合成与子宫颈中雌激素受体的变化无关；孕激素对雌激素受体系统的不同作用与同一动物两个靶组织内不同的形态学反应同时发生。
