Huang Zeng-Fang, Zhang Xi-Qi, Zhang Xiao-Yong, Yang Bin, Wang Ke, Wang Shi-Qi, Yuan Jin-Ying, Tao Lei, Wei Yen
School of Materials and Food Engineering, Zhongshan Institute, University of Electronic Science and Technology of China, Zhongshan, 528402, P. R. China.
Department of Chemistry, The Tsinghua Center for Frontier Polymer Research, Tsinghua University, Beijing 100084, P. R. China.
J Nanosci Nanotechnol. 2018 Apr 1;18(4):2345-2351. doi: 10.1166/jnn.2018.14287.
Due to the extensive source, good biocompatibility and biodegradability, the starch of carbohydrates has been extensively investigated for application in biological field. Recently, the development of fluorescent organic nanoparticles (FONs) on the basis of aggregation induced emission (AIE) dyes has attracted great research interest. In this article, novel starch-based S-TPEV polymers with AIE property were successfully fabricated by atom transfer radical polymerization (ATRP) of TPEV dye into water-soluble starch for the first time, subsequently, their structure and properties were detailedly investigated by 1H NMR, TEM, UV-vis, FL and FTIR. The characterization results confirmed the successful synthesis of S-TPEV polymers, and the molar fraction of TPEV and C6H10O5 ring in the starch polymers could be respectively calculated as approximate 5.8% and 94.2%. In aqueous solution, the as-prepared S-TPEV polymers will tend to self-assemble into FONs with 100-200 nm diameters, and their fluorescence intensity increased with the increase of the concentration of water in the mixed solution of water and DMSO, indicative of the obvious AIE property. More importantly, owing to their high water dispersity, good fluorescence and excellent biocompatibility, the S-TPEV FONs can be uptaken by HepG2 cells and show promising application in biological imaging field.
由于碳水化合物来源广泛、具有良好的生物相容性和生物降解性，碳水化合物中的淀粉已在生物领域的应用方面得到广泛研究。近年来，基于聚集诱导发光（AIE）染料的荧光有机纳米粒子（FONs）的开发引起了极大的研究兴趣。本文首次通过将TPEV染料原子转移自由基聚合（ATRP）到水溶性淀粉中，成功制备了具有AIE性质的新型淀粉基S-TPEV聚合物，随后，通过1H NMR、TEM、UV-vis、FL和FTIR对其结构和性能进行了详细研究。表征结果证实了S-TPEV聚合物的成功合成，淀粉聚合物中TPEV和C6H10O5环的摩尔分数分别可计算为约5.8%和94.2%。在水溶液中，所制备的S-TPEV聚合物倾向于自组装成直径为100-200 nm的FONs，并且它们的荧光强度随着水与DMSO混合溶液中水浓度的增加而增加，表明具有明显的AIE性质。更重要的是，由于其高水分散性、良好的荧光和优异的生物相容性，S-TPEV FONs可以被HepG2细胞摄取，并在生物成像领域显示出广阔的应用前景。
