Yu Shengxian, Xu Dazhuang, Wan Qing, Liu Meiying, Tian Jianwen, Huang Qiang, Deng Fengjie, Wen Yuanqing, Zhang Xiaoyong, Wei Yen
Department of Chemistry, Nanchang University, 999 Xuefu Avenue, Nanchang 330031, China.
Department of Chemistry, Nanchang University, 999 Xuefu Avenue, Nanchang 330031, China.
Mater Sci Eng C Mater Biol Appl. 2017 Sep 1;78:191-197. doi: 10.1016/j.msec.2017.04.064. Epub 2017 Apr 13.
Aggregation-induced emission (AIE) active fluorescence polymeric nanoparticles (FPNs) have recently received increasing interests for biomedical applications such as cell imaging, drug delivery, disease diagnosis and treatment. Fabricated strategies of AIE-active FPNs with high efficiency, simplification and tenderness are still passionately pursued to promote the development of theranostic systems. In this work, we develop a facile method for the preparation of AIE-active FPNs by adopting Ce(IV)/HNO redox polymerization under near room temperature. Thus-prepared FPNs (named as PEG-PLC-1) possess unique AIE feature, great water dispersity, excellent biocompatibility and biodegradability because of the conjugation of ultra-bright AIE dye (PhE-alc) and biodegradable PEG-PCL linear copolymers. The H nuclear magnetic resonance (NMR) spectroscopy, Fourier transform infrared (FT-IR) spectroscopy, transmission electron microscopy (TEM), UV-Visible and fluorescence spectrometers were used to confirm the successful fabrication of AIE-active FPNs. Cell viability and cellular uptake behavior of PEG-PLC-1 FPNs were further investigated for their potential biomedical applications. Results demonstrated that PEG-PLC-1 FPNs are high water dispersity, intensive luminescence and low cytotoxicity, making them very attractive for biomedical applications. More importantly, the method for the fabrication of AIE-active biodegradable FPNs can be occurred under rather facile conditions (e.g., low temperature, free of metal catalysts, common chain transfer agent and aqueous solution) and are specially used for fabrication of AIE-active polysaccharides with poor organic solubility.
聚集诱导发光（AIE）活性荧光聚合物纳米颗粒（FPNs）近来在细胞成像、药物递送、疾病诊断与治疗等生物医学应用方面受到越来越多的关注。为推动诊疗系统的发展，人们仍在积极探索高效、简便且温和的AIE活性FPNs制备策略。在本工作中，我们采用Ce(IV)/HNO氧化还原聚合反应在近室温条件下开发了一种制备AIE活性FPNs的简便方法。如此制备的FPNs（命名为PEG-PLC-1）由于超亮AIE染料（PhE-alc）与可生物降解的PEG-PCL线性共聚物的共轭作用，具有独特的AIE特性、良好的水分散性、优异的生物相容性和生物降解性。利用核磁共振氢谱（NMR）、傅里叶变换红外光谱（FT-IR）、透射电子显微镜（TEM）、紫外可见光谱仪和荧光光谱仪来确认AIE活性FPNs的成功制备。进一步研究了PEG-PLC-1 FPNs的细胞活力和细胞摄取行为以评估其潜在的生物医学应用。结果表明，PEG-PLC-1 FPNs具有高水分散性、强发光性和低细胞毒性，使其在生物医学应用中极具吸引力。更重要的是，制备AIE活性可生物降解FPNs的方法可以在相当简便的条件下（如低温、无金属催化剂、常用链转移剂和水溶液）进行，并且特别适用于制备有机溶解性差的AIE活性多糖。
