Shi Bowen, Lin Huimin, Zhang Miao, Lu Wei, Qu Ying, Zhang Huan
Department of Radiology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine.
Department of Nuclear Medicine, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine.
J Vis Exp. 2018 Jan 22(131):56526. doi: 10.3791/56526.
Gastric cancer remains fourth in cancer incidence worldwide with a five-year survival of only 20%-30%. Peritoneal metastasis is the most frequent type of metastasis that accompanies unresectable gastric cancer and is a definitive determinant of prognosis. Preventing and controlling the development of peritoneal metastasis could play a role in helping to prolong the survival of gastric cancer patients. A non-invasive and efficient imaging technique will help us to identify the invasion and metastasis process of peritoneal metastasis and to monitor the changes in tumor nodules in response to treatments. This will enable us to obtain an accurate description of the development process and molecular mechanisms of gastric cancer. We have recently described experiment using dual energy CT (DECT) and positron emission tomography/computed tomography (PET/CT) platforms for the detection and monitoring of gastric tumor metastasis in nude mice models. We have shown that weekly continuous monitoring with DECT and PET/CT can identify dynamic changes in peritoneal metastasis. The sFRP1-overexpression in gastric cancer mice models showed positive radiological performance, a higher FDG uptake and increasing enhancement, and the SUVmax (standardized uptake value) of nodules demonstrated an obvious alteration trend in response to targeted therapy of TGF-β1 inhibitor. In this article, we described the detailed non-invasive imaging procedures to conduct more complex research on gastric cancer peritoneal metastasis using animal models and provided representative imaging results. The use of non-invasive imaging techniques should enable us to better understand the mechanisms of tumorigenesis, monitor tumor growth, and evaluate the effect of therapeutic interventions for gastric cancer.
胃癌的全球癌症发病率仍位居第四，其五年生存率仅为20%-30%。腹膜转移是不可切除胃癌最常见的转移类型，也是预后的决定性因素。预防和控制腹膜转移的发展可能有助于延长胃癌患者的生存期。一种非侵入性且高效的成像技术将有助于我们识别腹膜转移的侵袭和转移过程，并监测肿瘤结节对治疗的反应变化。这将使我们能够准确描述胃癌的发展过程和分子机制。我们最近描述了使用双能CT（DECT）和正电子发射断层扫描/计算机断层扫描（PET/CT）平台在裸鼠模型中检测和监测胃肿瘤转移的实验。我们已经表明，每周使用DECT和PET/CT进行连续监测可以识别腹膜转移的动态变化。胃癌小鼠模型中的sFRP1过表达显示出阳性的放射学表现、更高的FDG摄取和增强增加，并且结节的SUVmax（标准化摄取值）在TGF-β1抑制剂的靶向治疗后呈现出明显的变化趋势。在本文中，我们描述了详细的非侵入性成像程序，以便使用动物模型对胃癌腹膜转移进行更复杂的研究，并提供了代表性的成像结果。使用非侵入性成像技术应该使我们能够更好地理解肿瘤发生的机制，监测肿瘤生长，并评估胃癌治疗干预的效果。
