School of Food Science and Engineering , South China University of Technology , Guangzhou 510641 , China.
Academy of Contemporary Food Engineering , South China University of Technology, Guangzhou Higher Education Mega Center , Guangzhou 510006 , China.
J Agric Food Chem. 2018 Mar 7;66(9):2180-2187. doi: 10.1021/acs.jafc.7b05664. Epub 2018 Feb 22.
A simple method based on surface-enhanced Raman scattering (SERS) was developed for the rapid determination of alternariol (AOH) in pear fruits using an easily prepared silver-nanoparticle (AgNP) substrate. The AgNP substrate was modified by pyridine to circumvent the weak affinity of the AOH molecules to the silver surface and to improve the sensitivity of detection. Quantitative analysis was performed in AOH solutions at concentrations ranging from 3.16 to 316.0 μg/L, and the limit of detection was 1.30 μg/L. The novel method was also applied to the detection of AOH residues in pear fruits purchased from the market and in pear fruits that were artificially inoculated with Alternaria alternata. AOH was not found in any of the fresh fruit, whereas it resided in the rotten and inoculated fruits. Finally, the SERS method was cross validated against HPLC. It was revealed that the SERS method has great potential utility in the rapid detection of AOH in pear fruits and other agricultural products.
一种基于表面增强拉曼散射（SERS）的简单方法被开发出来，用于使用易于制备的银纳米粒子（AgNP）基底快速测定梨果中的交替曲酸（AOH）。AgNP 基底用吡啶修饰，以避免 AOH 分子与银表面的弱亲和力，并提高检测的灵敏度。在浓度范围为 3.16 至 316.0 μg/L 的 AOH 溶液中进行定量分析，检测限为 1.30 μg/L。该新方法还应用于检测市场上购买的梨果和人工接种链格孢菌的梨果中的 AOH 残留。新鲜水果中均未发现 AOH，而腐烂和接种的水果中则含有 AOH。最后，SERS 方法与 HPLC 进行了交叉验证。结果表明，SERS 方法在快速检测梨果和其他农产品中的 AOH 方面具有很大的应用潜力。
