Giryn H, Szteke B
Zakładu Analizy Zywności Instytutu Biotechnologii Przemsłu Rołno-Spozywczego w Warszawie.
Rocz Panstw Zakl Hig. 1995;46(2):129-33.
The purpose of the study was the assessment of Alternaria-mycotoxins contamination in some raw and processed plant products. The analytical method for detection of alternariol (AOH) and alternariol methyl ether (AME) is described. After extraction and purification of sample crude extracts by column chromatography on silica gel, the qualitative and quantitative analyses were carried out by two-dimensional TLC. There were 110 samples analyzed -44 (included 32 moulded) raw plant samples and 56 processed plant products. Levels of alternaria-mycotoxins found in fruits and tomatoes visibly decayed ranged between 3 to 420 micrograms/kg for AOH and 10 to 100 micrograms/kg for AME. Trace amounts of AOH were detected in 3 samples of processed products.
本研究的目的是评估某些生鲜及加工植物产品中的链格孢霉菌毒素污染情况。文中描述了检测交链孢酚(AOH)和交链孢酚单甲醚(AME)的分析方法。通过硅胶柱色谱法对样品粗提物进行萃取和纯化后,采用二维薄层色谱法进行定性和定量分析。共分析了110个样品,其中44个(包括32个发霉的)生鲜植物样品和56个加工植物产品。在明显腐烂的水果和番茄中发现的链格孢霉菌毒素水平,AOH为3至420微克/千克,AME为10至100微克/千克。在3个加工产品样品中检测到痕量的AOH。