脂肪干细胞接种丝素蛋白壳聚糖膜对糖尿病大鼠模型伤口愈合影响的实验研究
Experimental Study on Effects of Adipose-Derived Stem Cell-Seeded Silk Fibroin Chitosan Film on Wound Healing of a Diabetic Rat Model.
作者信息
Wu Yan-Yun, Jiao Yan-Peng, Xiao Li-Ling, Li Min-Min, Liu Hong-Wei, Li Sheng-Hong, Liao Xuan, Chen Yong-Tian, Li Jiang-Xuan, Zhang Yang
出版信息
Ann Plast Surg. 2018 May;80(5):572-580. doi: 10.1097/SAP.0000000000001355.
BACKGROUND
Wound healing is a complex process that relies on growth factors and stimulation of angiogenesis. Tissue engineering materials composed of adipose-derived stem cells (ADSCs) and silk fibroin (SF)/chitosan (CS) may be able to solve this problem. The aim of this study was to investigate the wound-healing potential of ADSC-seeded SF/CS in streptozotocin-induced diabetic rats.
MATERIALS AND METHODS
Thirty-six male Sprague-Dawley rats were purchased and randomly assigned into 3 groups: a control group (no graft), a group treated with SF/CS film graft, and a group treated with ADSC-seeded SF/CS graft. The number of animals in each group was 12. Diabetes was induced by an intraperitoneal injection of streptozotocin. A cutaneous wound was incised at the dorsal region of all the experimental animals. The ADSCs were labeled with CM-Dil fluorescent staining. Wound healing was assessed for all animal groups by observing the rate of wound closure and hematoxylin and eosin staining. The expression of epidermal growth factor, transforming growth factor-β, and vascular endothelial growth factor at the wound sites was studied by enzyme-linked immunosorbent assay to evaluate the effect of growth factors secreted by ADSCs. The differentiation of ADSCs was analyzed by immunofluorescence staining.
RESULTS
The ADSC-seeded SF/CS film treatment significantly increased the rates of wound closure in treated animals, and hence wound healing was drastically enhanced for ADSC-SF/CS treatment groups compared with control groups and SF/CS film treatment group. Histological observations showed the condition of wound healing. Enzyme-linked immunosorbent assay and immunofluorescence staining observations showed the secretion and differentiation of ADSCs, respectively.
CONCLUSIONS
Our analyses clearly suggested that it is feasible and effective to enhance wound healing in a diabetic rat model with ADSC-seeded SF/CS film.
背景
伤口愈合是一个复杂的过程，依赖于生长因子和血管生成的刺激。由脂肪来源干细胞（ADSCs）与丝素蛋白（SF）/壳聚糖（CS）组成的组织工程材料或许能够解决这一问题。本研究的目的是探讨接种ADSCs的SF/CS在链脲佐菌素诱导的糖尿病大鼠中的伤口愈合潜力。
材料与方法
购买36只雄性Sprague-Dawley大鼠并随机分为3组：对照组（未移植）、接受SF/CS膜移植治疗的组和接受接种ADSCs的SF/CS移植治疗的组。每组动物数量为12只。通过腹腔注射链脲佐菌素诱导糖尿病。在所有实验动物的背部区域切开一个皮肤伤口。用CM-Dil荧光染色标记ADSCs。通过观察伤口闭合率和苏木精-伊红染色对所有动物组的伤口愈合情况进行评估。通过酶联免疫吸附测定研究伤口部位表皮生长因子、转化生长因子-β和血管内皮生长因子的表达，以评估ADSCs分泌的生长因子的作用。通过免疫荧光染色分析ADSCs的分化情况。
结果
接种ADSCs的SF/CS膜治疗显著提高了治疗动物的伤口闭合率，因此与对照组和SF/CS膜治疗组相比，ADSC-SF/CS治疗组的伤口愈合得到显著增强。组织学观察显示了伤口愈合情况。酶联免疫吸附测定和免疫荧光染色观察分别显示了ADSCs的分泌和分化情况。
结论
我们的分析清楚地表明，用接种ADSCs的SF/CS膜增强糖尿病大鼠模型中的伤口愈合是可行且有效的。
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