State Key Joint Laboratory of Environmental Simulation and Pollution Control, College of Environmental Sciences and Engineering, Peking University, Beijing 100871, China.
Key Laboratory of Water and Sediment Sciences (Ministry of Education), College of Environmental Sciences and Engineering, Peking University, Beijing 100871, China.
Sci Total Environ. 2018 Jul 1;628-629:310-318. doi: 10.1016/j.scitotenv.2018.02.041. Epub 2018 Feb 13.
Ammonia oxidation, performed by both ammonia oxidizing bacteria (AOB) and archaea (AOA), is an important step for nitrogen removal in constructed wetlands (CWs). However, little is known about the distribution of these ammonia oxidizing organisms in CWs and the associated wetland environmental variables. Their relative importance to nitrification in CWs remains still controversial. The present study investigated the seasonal dynamics of AOA and AOB communities in a free water surface flow CW (FWSF-CW) used to ameliorate the quality of polluted river water. Strong seasonality effects on potential nitrification rate (PNR) and the abundance, richness, diversity and structure of AOA and AOB communities were observed in the river water treatment FWSF-CW. PNR was positively correlated to AOB abundance. AOB (6.76×10-6.01×10 bacterial amoA gene copies per gram dry sediment/soil) tended to be much more abundant than AOA (from below quantitative PCR detection limit to 9.62×10 archaeal amoA gene copies per gram dry sediment/soil). Both AOA and AOB abundance were regulated by the levels of nitrogen, phosphorus and organic carbon. Different wetland environmental variables determined the diversity and structure of AOA and AOB communities. Wetland AOA communities were mainly composed of unknown species and Nitrosopumilus-like organisms, while AOB communities were mainly represented by both Nitrosospira and Nitrosomonas.
氨氧化作用由氨氧化细菌（AOB）和古菌（AOA）共同完成，是人工湿地（CWs）中脱氮的重要步骤。然而，人们对这些氨氧化生物在 CWs 中的分布以及相关湿地环境变量知之甚少。它们对 CWs 中硝化作用的相对重要性仍然存在争议。本研究调查了用于改善受污染河水水质的自由水面流动人工湿地（FWSF-CW）中氨氧化古菌（AOA）和氨氧化细菌（AOB）群落的季节性动态。在河水处理 FWSF-CW 中，观察到潜在硝化速率（PNR）以及 AOA 和 AOB 群落的丰度、丰富度、多样性和结构具有强烈的季节性变化。PNR 与 AOB 丰度呈正相关。AOB（6.76×10-6.01×10 个细菌 amoA 基因拷贝数/克干沉积物/土壤）的丰度明显高于 AOA（从定量 PCR 检测下限到 9.62×10 个古菌 amoA 基因拷贝数/克干沉积物/土壤）。AOA 和 AOB 的丰度均受氮、磷和有机碳水平的调节。不同的湿地环境变量决定了 AOA 和 AOB 群落的多样性和结构。湿地 AOA 群落主要由未知物种和 Nitrosopumilus 样生物组成，而 AOB 群落主要由 Nitrosospira 和 Nitrosomonas 组成。
