College of Marine and Civil Engineering, Dalian Ocean University, Dalian 116023, China.
Guangdong Provincial Key Laboratory of Environmental Pollution Control and Remediation Technology, Sun Yat-sen University, Guangzhou 510275, China.
Int J Environ Res Public Health. 2019 Mar 5;16(5):802. doi: 10.3390/ijerph16050802.
Wetland plants that cover the wetlands play an important role in reducing pollutants. The aim of this study was to investigate the effect of two plant species on microbial communities and nitrogen-removal genes and to evaluate the contributions of absorbing pollutants by (CI) and (CA) to the removal performance in both a vertical subsurface flow constructed wetland and a horizontal subsurface flow constructed wetland, which were part of a full-scale hybrid constructed wetland system. The microbial assemblages were determined using 16S rRNA high-throughput sequencing. Results showed that the presence of CI and CA positively affected microbial abundance and community in general and which was positive for the total bacteria and ammonia nitrogen removal in the CWs. The higher abundance of Nitrospirae appeared in the non-rhizosphere sediment (NRS) than that in the rhizosphere sediment (RS). More denitrification genes were found in NRS than in RS. The copy numbers of , and genes for CA were higher than those for CI. Wetland plant species can significantly ( < 0.05) affect the distribution of microbial communities in RS. Plant selection is important to promote the development of microbial communities with a more active and diverse catabolic capability and the contribution of plant absorption to the overall removal rate of wetland system can be neglected.
湿地中覆盖的湿地植物在减少污染物方面发挥着重要作用。本研究旨在探讨两种植物物种对微生物群落和脱氮基因的影响，并评估在垂直潜流人工湿地和水平潜流人工湿地中，通过吸收污染物（CI）和（CA）对去除性能的贡献，这两个湿地是全规模混合人工湿地系统的一部分。使用 16S rRNA 高通量测序来确定微生物组合。结果表明，CI 和 CA 的存在总体上对微生物丰度和群落有积极影响，这对 CWs 中的总细菌和氨氮去除有积极影响。硝态螺旋菌在非根际沉积物（NRS）中的丰度高于根际沉积物（RS）中的丰度。NRS 中发现的脱氮基因多于 RS 中的脱氮基因。CA 的 、和基因的拷贝数高于 CI。湿地植物物种可以显著（<0.05）影响 RS 中微生物群落的分布。植物选择对于促进具有更活跃和多样化的代谢能力的微生物群落的发展很重要，并且可以忽略植物吸收对湿地系统整体去除率的贡献。
