Jinzhou Medical University, Jinzhou, 121001, People's Republic of China.
Int J Nanomedicine. 2018 Jun 18;13:3493-3506. doi: 10.2147/IJN.S157463. eCollection 2018.
Treatment of infection within bone is difficult, and conventional surgical treatment brings intense pain to the patients physically and mentally. There is an urgent need to develop injectable nano- and/or micro-medicine for minimally invasive treatment of osteomyelitis.
In this paper, amino acid (L-lysine [Lys]) was mineralized into yolk-shell structured CaCO microspheres (MSs). The morphologies of the obtained MSs were investigated by scanning electron microscopy and transmission electron microscopy. The composition of CaCO MSs was identified by using Fourier transform infrared spectroscopy. The as-prepared CaCO MSs were examined with power X-ray diffraction analysis to obtain the crystallographic structure of the MSs.
The as prepared Lys encapsulated CaCO MSs (Lys@CaCO MSs) were used as micro-drug to improve acidic environment of osteomyelitis caused by bacterial infection and promote osteoblast proliferation under oxidative stress. These pH responsive Lys@CaCO MSs have a drug loading efficiency of 89.8 wt % and drug loading content (DLC) of 22.3 wt %.
Our results demonstrated that Lys@CaCO MSs can effectively kill and promote proliferation and differentiation of osteoblasts under stimulation of HO at pH = 5.5.
骨内感染的治疗较为困难，常规的外科治疗给患者带来了极大的身心痛苦。因此，急需开发可注射的纳米和/或微药物，以实现微创治疗骨髓炎。
本文将氨基酸（L-赖氨酸[Lys]）矿化形成蛋黄壳结构的 CaCO 微球（MSs）。通过扫描电子显微镜和透射电子显微镜研究了所得 MSs 的形态。通过傅里叶变换红外光谱鉴定了 CaCO MSs 的组成。采用粉末 X 射线衍射分析对所制备的 CaCO MSs 进行了晶体结构分析。
所制备的 Lys 包封的 CaCO MSs（Lys@CaCO MSs）被用作微药物，以改善细菌感染引起的骨髓炎的酸性环境，并在氧化应激下促进成骨细胞的增殖。这些 pH 响应型 Lys@CaCO MSs 的载药效率为 89.8 wt%，载药量（DLC）为 22.3 wt%。
我们的结果表明，Lys@CaCO MSs 在 pH = 5.5 下的 HO 刺激下，可以有效杀死细菌并促进成骨细胞的增殖和分化。
