Wolter H J
Brain Res. 1986 Aug 27;381(1):121-4. doi: 10.1016/0006-8993(86)90698-0.
Using the microdissection technique combined with the pre-embedding immunostaining method, beta-endorphin immunoreactivity (beta-END-IR) was noted within ring-shaped elements lying singly or in groups in nerve processes of the duodenal myenteric plexus of the rat. The beta-END-IR was noted as a strongly electron-dense precipitate rim on the inner surface of these elements. The membrane of the ring-shaped element as well as the beta-END precipitate rim were strikingly often unilaterally interrupted at the same site. Some of the ring-shaped beta-END-positive elements were noted within large osmiophilic bodies. The beta-END immunoreactive ring structure was 60-300 nm in diameter and possessed an empty electron-lucent space as well as a thin electron-dense membrane. The osmiophilic bodies were pleormorph, had a mean diameter of 250 nm and revealed thick electron-opaque membranes. Their inner spaces were homogenously filled with electron-dense materials. These findings might indicate that the beta-END-IR-containing ring-shaped elements represent receptosomes and that the pleomorph osmiophilic bodies are identical to lysosomes.
采用显微解剖技术结合包埋前免疫染色方法，在大鼠十二指肠肌间神经丛神经突起中单个或成群分布的环形结构内发现了β-内啡肽免疫反应性（β-END-IR）。β-END-IR表现为这些结构内表面上强烈电子致密的沉淀边缘。环形结构的膜以及β-END沉淀边缘常常在同一部位出现单侧中断。在大的嗜锇性小体内发现了一些β-END阳性的环形结构。β-END免疫反应性环形结构直径为60 - 300 nm，具有一个空的电子透明空间以及一层薄的电子致密膜。嗜锇性小体形态多样，平均直径为250 nm，显示出厚的电子不透明膜。其内部空间均匀地填充有电子致密物质。这些发现可能表明含β-END-IR的环形结构代表受体小体，而形态多样的嗜锇性小体与溶酶体相同。
