Soleimani Asl Sara, Hesam Shariati Mohamad Bakhtiar, Medizadeh Mehdi, Ahmadpanah Mohammad, Sohrabi Maryam
Research Center for Behavioral Disorders and Substance Abuse, Hamadan University of Medical Sciences, Hamadan, Iran.
Anatomy Department, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
Med J Islam Repub Iran. 2017 Sep 10;31:60. doi: 10.14196/mjiri.31.60. eCollection 2017.
3,4- methylenedioxymethamphetamine (MDMA) is a chemical derivative of amphetamine that can induce learning and memory impairment. Due to the effect of neurotrophins on memory and learning, the impact of MDMA was evaluated on the brain - derived neurotrophic factor (BDNF), neurotrophin- 4 (NT-4), and tropomyosin- related kinase B (Trk- β) expression in the hippocampus. In this study, 20 adult male Wistar rats (200-250 g) received saline (1 mL) or 10 mg/kg of MDMA intraperitoneally as single or multiple injection for 2 consecutive days per week for 2 months. Expression of BDNF, Trk-β, and NT4 were assessed using Western blotting and RT PCR methods. Our results revealed that the expression of BDNF, Trk- β, and NT4 proteins and genes significantly decreased in MDMA groups compared to the sham group (p<0.05). Furthermore, the acute group showed the lowest expression of these proteins. The results of the present study suggest that ecstasy administration may downregulate the expression of BDNF, Trk- β, and NT-4 in hippocampus, which is more extensive in case of acute treatment. It seems that in the chronic group, hippocampus was able to compensate the ecstasy- induced neurotoxicity.
3,4-亚甲基二氧甲基苯丙胺（摇头丸）是苯丙胺的一种化学衍生物，可导致学习和记忆障碍。由于神经营养因子对记忆和学习有影响，因此评估了摇头丸对海马体中脑源性神经营养因子（BDNF）、神经营养因子4（NT-4）和原肌球蛋白相关激酶B（Trk-β）表达的影响。在本研究中，20只成年雄性Wistar大鼠（200-250克）每周连续2天腹腔注射生理盐水（1毫升）或10毫克/千克摇头丸，共注射2个月。使用蛋白质免疫印迹法和逆转录聚合酶链反应（RT-PCR）方法评估BDNF、Trk-β和NT4的表达。我们的结果显示，与假手术组相比，摇头丸组中BDNF、Trk-β和NT4蛋白及基因的表达显著降低（p<0.05）。此外，急性组中这些蛋白的表达最低。本研究结果表明，服用摇头丸可能会下调海马体中BDNF、Trk-β和NT-4的表达，急性治疗时这种下调更为明显。似乎在慢性组中，海马体能够代偿摇头丸诱导的神经毒性。
