Ewald Collin Yvès, Hourihan John M, Blackwell T Keith
Department of Health Sciences and Technology, Eidgenössische Technische Hochschule (ETH) Zürich, Schwerzenbach-Zürich, Switzerland.
Department of Genetics, Harvard Medical School, Boston, Massachusetts, USA.
Bio Protoc. 2017 Jul 5;7(13). doi: 10.21769/BioProtoc.2365.
Cells and organisms face constant exposure to reactive oxygen species (ROS), either from the environment or as a by-product from internal metabolic processes. To prevent cellular damage from ROS, cells have evolved detoxification mechanisms. The activation of these detoxification mechanisms and their downstream responses represent an overlapping defense response that can be tailored to different sources of ROS to adequately adapt and protect cells. In this protocol, we describe how to measure the sensitivity to oxidative stress from two different sources, arsenite and tBHP, using the nematode .
细胞和生物体不断暴露于活性氧（ROS）中，这些ROS要么来自环境，要么是内部代谢过程的副产物。为了防止ROS对细胞造成损伤，细胞进化出了解毒机制。这些解毒机制的激活及其下游反应代表了一种重叠的防御反应，可根据不同的ROS来源进行调整，以充分适应和保护细胞。在本实验方案中，我们描述了如何使用线虫来测量对两种不同来源的氧化应激——亚砷酸盐和叔丁基过氧化氢的敏感性。
