Department of Chemical Engineering, Ryerson University, 350 Victoria Street, Toronto, M5B 2K3, Canada.
Sanofi Pasteur Company, 1755 Steels Avenue West, North York, Toronto, M2R 3T4, Canada.
Bioprocess Biosyst Eng. 2018 May;41(5):679-695. doi: 10.1007/s00449-018-1902-7. Epub 2018 Feb 14.
The hydrodynamics of gas-liquid two-phase flow in a single-use bioreactor were investigated in detail both experimentally and numerically. Electrical resistance tomography (ERT) and dynamic gas disengagement (DGD) combined with computational fluid dynamics (CFD) were employed to assess the effect of the volumetric gas flow rate and impeller speed on the gas-liquid flow field, local and global gas holdup values, and Sauter mean bubble diameter. From the results obtained from DGD coupled with ERT, the bubble sizes were determined. The experimental data indicated that the total gas holdup values increased with increasing both the rotational speed of impeller and volumetric gas flow rate. Moreover, the analysis of the flow field generated inside the aerated stirred bioreactor was conducted using CFD results. Overall, a more uniform distribution of the gas holdup was obtained at impeller speeds ≥ 100 rpm for volumetric gas flow rates ≥ 1.6 × 10 m/s.
在一次性生物反应器中，通过实验和数值研究详细考察了气液两相流的流体动力学。电阻层析成像（ERT）和动态气体分离（DGD）与计算流体动力学（CFD）相结合，用于评估体积气体流量和搅拌器速度对气液流场、局部和整体气含率以及索太尔平均气泡直径的影响。从 DGD 与 ERT 结合获得的结果中确定了气泡尺寸。实验数据表明，总气含率随着搅拌器转速和体积气体流量的增加而增加。此外，使用 CFD 结果对充气搅拌生物反应器内部产生的流场进行了分析。总的来说，在体积气体流量≥1.6×10^-3 m/s 时，搅拌器速度≥100 rpm 时可以获得更均匀的气含率分布。
