Department of Horticulture, Zhejiang University, 866 Yuhangtang Road, 310058, Hangzhou, People's Republic of China.
Experimental Station of Zhejiang University, 866 Yuhangtang Road, 310058, Hangzhou, People's Republic of China.
Planta. 2018 May;247(5):1217-1227. doi: 10.1007/s00425-018-2860-7. Epub 2018 Feb 14.
N-decanoyl-homoserine lactone activates plant systemic resistance against Botrytis cinerea in tomato plants, which is largely dependent on jasmonic acid biosynthesis and signal transduction pathways. Rhizosphere bacteria secrete N-acylated-homoserine lactones (AHLs), a type of specialized quorum-sensing signal molecule, to coordinate their population density during communication with their eukaryotic hosts. AHLs behave as low molecular weight ligands that are sensed by plants and promote the host's resistance against foliar pathogens. In this study, we report on N-decanoyl-homoserine lactone (DHL), which is a type of AHL that induces systemic immunity in tomato plants and protects the host organism against the necrotrophic fungus Botrytis cinerea. Upon DHL treatment, tomato endogenous jasmonic acid (JA) biosynthesis (rather than salicylic acid biosynthesis) and signal transduction were significantly activated. Strikingly, the DHL-induced systemic resistance against B. cinerea was blocked in the tomato JA biosynthesis mutant spr2 and JA signaling gene-silenced plants. Our findings highlight the role of DHL in systemic resistance against economically important necrotrophic pathogens and suggest that DHL-induced immunity against B. cinerea is largely dependent on the JA signaling pathway. Manipulation of DHL-induced resistance is an attractive disease management strategy that could potentially be used to enhance disease resistance in diverse plant species.
壬酰基高丝氨酸内酯激活番茄植株对灰葡萄孢的系统性抗性，而这在很大程度上依赖于茉莉酸生物合成和信号转导途径。根际细菌分泌 N-酰基高丝氨酸内酯（AHLs），这是一种专门的群体感应信号分子，用于在与真核宿主的交流中协调其种群密度。AHLs 作为低分子量配体被植物感知，并促进宿主对叶部病原体的抗性。在这项研究中，我们报告了壬酰基高丝氨酸内酯（DHL），它是一种诱导番茄植株系统性免疫并保护宿主免受坏死真菌灰葡萄孢侵害的 AHL。在 DHL 处理后，番茄内源茉莉酸（JA）生物合成（而非水杨酸生物合成）和信号转导显著激活。引人注目的是，在番茄 JA 生物合成突变体 spr2 和 JA 信号基因沉默植物中，DHL 诱导的对灰葡萄孢的系统性抗性被阻断。我们的研究结果强调了 DHL 在系统性抵抗经济上重要的坏死性病原体中的作用，并表明 DHL 诱导的对灰葡萄孢的抗性在很大程度上依赖于 JA 信号通路。操纵 DHL 诱导的抗性是一种有吸引力的疾病管理策略，可能用于增强多种植物物种的抗病性。
