College of Food Science and Nutritional Engineering , China Agricultural University , Beijing 100083 , China.
School of Agricultural Economics and Rural Development , Renmin University of China , Beijing 100872 , China.
J Agric Food Chem. 2018 Aug 29;66(34):8949-8956. doi: 10.1021/acs.jafc.8b02191. Epub 2018 Aug 20.
Mitogen-activated protein kinases (MAPKs) play an important role in defense responses to biotic and abiotic stresses. In order to investigate the role of SlMAPK3 in tomato plant resistance to Botrytis cinerea, two lines of slmapk3 mutants and wild-type (WT) tomato plants were used. The results showed that slmapk3 mutants were more susceptible to B. cinerea and that knockout of SlMAPK3 reduced the activities of defense enzymes and enhanced the accumulation of reactive oxygen species (ROS). Furthermore, we detected the expressions of salicylic acid (SA) and jasmonic acid (JA) signaling-related genes and found that knockout of SlMAPK3 enhanced the expressions of SlPR1, SlPAD4 and SlEDS1, whereas reduced the expressions of SlLoxC, SlPI I and SlPI II and enhanced the expressions of SlJAZ1 and SlMYC2. We postulate that SlMAPK3 plays a positive role in tomato plant resistance to B. cinerea through regulating ROS accumulation and SA and JA defense signaling pathways.
丝裂原活化蛋白激酶（MAPKs）在生物和非生物胁迫的防御反应中发挥重要作用。为了研究 SlMAPK3 在番茄植株对灰葡萄孢抗性中的作用，使用了两条 slmapk3 突变体和野生型（WT）番茄植株。结果表明，slmapk3 突变体对灰葡萄孢更加敏感，并且 SlMAPK3 的敲除降低了防御酶的活性并增强了活性氧（ROS）的积累。此外，我们检测了水杨酸（SA）和茉莉酸（JA）信号相关基因的表达，发现 SlMAPK3 的敲除增强了 SlPR1、SlPAD4 和 SlEDS1 的表达，而降低了 SlLoxC、SlPI I 和 SlPI II 的表达，并增强了 SlJAZ1 和 SlMYC2 的表达。我们推测 SlMAPK3 通过调节 ROS 积累和 SA 和 JA 防御信号通路在番茄植株对灰葡萄孢的抗性中发挥积极作用。
