Cuttance E L, Laven R A, Watts A
a VetEnt Research , 49 Benson Road, Te Awamutu , New Zealand.
b School of Veterinary Science , Massey University , Palmerston North , 4410 , New Zealand.
N Z Vet J. 2018 Jul;66(4):194-198. doi: 10.1080/00480169.2018.1440653. Epub 2018 Mar 1.
To determine the impact of sodium molybdate treatment, given weekly, on concentrations of Cu in liver, activity of liver enzymes, and weight gain over 4 weeks, in yearling bulls with elevated concentrations of Cu in liver.
The study was carried on two commercial grazing farms in the Otago region of New Zealand in yearling Friesian bulls (n=150 on Farm A and n=49 on Farm B) with mean concentration of Cu in liver >3,000 µmol/kg fresh weight. On Day 0, all animals were weighed and half were systematically allocated to treatment with sodium molybdate (3 mg/kg liveweight on Farm A and 7 mg/kg liveweight on Farm B); the remainder received no treatment (Control). Sodium molybdate was given as a drench weekly for 4 weeks and all animals were weighed again on Day 28. Ten animals on each farm (five from each treatment group) were systematically selected for blood sampling and liver biopsies on Days 0 and 28. Samples were analysed for concentrations of Cu in plasma, vitamin B in serum, activities of γ-glutamyl transferase, aspartate aminotransferase and glutamate dehydrogenase in serum, and concentrations of Cu and vitamin B in liver. Separate multivariable linear models were used to compare the change in outcome variables between Days 0 and 28 between bulls that had been drenched with sodium molybdate or not.
On Farm A, mean concentrations of Cu in liver on Day 28, as a percentage of concentrations on Day 0, for the control group was 55 (95% CI=40-73)% and for the treatment group was 73 (95% CI=43-111)%. On Farm B, the equivalent mean for the control group was 75 (95% CI=42-131)% and for the treatment group was 85 (95% CI=38-134)%. The multivariable linear models indicated that the changes in concentrations of Cu in liver, activities of liver enzymes and weight gain between Days 0 and 28 did not differ between the bulls treated or not with sodium molybdate (p>0.18).
Treatment with sodium molybdate in one bolus at weekly intervals for 4 weeks did not affect concentrations of Cu in liver, activity of liver enzymes or weight gain in animals with high concentrations of Cu liver on two farms.
确定每周给予钼酸钠治疗对肝脏铜浓度、肝脏酶活性以及肝脏铜浓度升高的一岁公牛在4周内体重增加的影响。
该研究在新西兰奥塔哥地区的两个商业放牧农场进行，对象为一岁的弗里西亚公牛（农场A有150头，农场B有49头），其肝脏铜的平均浓度>3,000微摩尔/千克鲜重。在第0天，对所有动物进行称重，然后将一半动物系统地分配到钼酸钠治疗组（农场A为3毫克/千克体重，农场B为7毫克/千克体重）；其余动物不接受治疗（对照组）。钼酸钠每周灌服一次，持续4周，所有动物在第28天再次称重。在第0天和第28天，从每个农场系统地挑选10头动物（每个治疗组5头）进行采血和肝脏活检。分析样本中的血浆铜浓度、血清维生素B、血清γ-谷氨酰转移酶、天冬氨酸氨基转移酶和谷氨酸脱氢酶活性以及肝脏铜和维生素B浓度。使用单独的多变量线性模型比较在第0天和第28天之间，接受或未接受钼酸钠灌服的公牛之间结局变量的变化。
在农场A，第28天肝脏铜的平均浓度相对于第0天浓度的百分比，对照组为55（95%可信区间=40-73）%，治疗组为73（95%可信区间=43-111）%。在农场B，对照组的相应平均值为75（95%可信区间=42-131）%，治疗组为85（95%可信区间=38-134）%。多变量线性模型表明，在第0天和第28天之间，接受或未接受钼酸钠治疗的公牛在肝脏铜浓度、肝脏酶活性和体重增加方面的变化没有差异（p>0.18）。
在两个农场中，每周一次连续4周给予钼酸钠治疗，对肝脏铜浓度高的动物的肝脏铜浓度、肝脏酶活性或体重增加没有影响。
