CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, U1019 - UMR 8204 - CIIL - Centre d'Infection et d'Immunité de Lille, Univ. Lille, Lille, France.
Cell Microbiol. 2018 Jun;20(6):e12832. doi: 10.1111/cmi.12832. Epub 2018 Mar 13.
Toxoplasma gondii is a unicellular eukaryotic pathogen that belongs to the Apicomplexa phylum, which encompasses some of the deadliest pathogens of medical and veterinary importance. The centrosome is key to the organisation and coordination of the cell cycle and division of apicomplexan parasites. The T. gondii centrosome possesses a particular bipartite structure (outer and inner cores). One of the main roles of the centrosome is to ensure proper coordination of karyokinesis. However, how these 2 events are coordinated is still unknown in T. gondii, for which the centrosome components are poorly described. To gain more insights into the biology and the composition of the T. gondii centrosome, we characterised a protein that resides at the interface of the outer and inner core centrosomes. TgCep530 is a large coiled-coil protein with an essential role in the survival of the parasite. Depletion of this protein leads to the accumulation of parasites lacking nuclei and disruption of the normal cell cycle. Lack of TgCep530 results in a discoordination between the nuclear cycle and the budding cycle that yields fully formed parasites without nuclei. TgCep530 has a crucial role in the coordination of karyokinesis and cytokinesis.
刚地弓形虫是一种单细胞真核病原体，属于顶复门，其中包括一些对医学和兽医具有重要意义的最致命病原体。中心体是细胞周期和分裂的组织和协调的关键，顶复门寄生虫。弓形虫的中心体具有独特的二部分结构（外核和内核）。中心体的主要作用之一是确保有丝分裂的正确协调。然而，在顶复门寄生虫中，这两个事件是如何协调的仍然未知，因为中心体的成分描述得很差。为了更深入地了解刚地弓形虫的生物学和中心体的组成，我们对位于外核和内核中心体界面的一种蛋白质进行了表征。TgCep530 是一种大型卷曲螺旋蛋白，在寄生虫的存活中起着重要作用。该蛋白的缺失会导致缺乏细胞核的寄生虫积累，并破坏正常的细胞周期。缺乏 TgCep530 会导致核周期和出芽周期之间失去协调，从而产生完全没有细胞核的成熟寄生虫。TgCep530 在有丝分裂和胞质分裂的协调中起着关键作用。
