Ghasemi Sorayya, Lorigooini Zahra, Wibowo JokoPriyanto, Amini-Khoei Hossein
a Cellular and Molecular Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences , Shahrekord , Iran.
b Medicinal Plants Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences , Shahrekord , Iran.
Nat Prod Res. 2019 Jun;33(12):1828-1831. doi: 10.1080/14786419.2018.1437439. Epub 2018 Feb 15.
For more effectiveness and overcoming the drug resistance Chemotherapy agents, the combination treatment is raised. Flavonoids with different anti-cancer effects are an appropriate choice as lead compounds. Over expressed MiR-21 in prostate cancer is associated with metastasis and drug resistance to chemotherapy with Docetaxel. In this study, the anticancer effect of 4', 5, 7-Trihydroxy-3', 5'-dimethoxyflavone (Tricin) was investigated with Docetaxel on PC3 cell line. Tricin was initially isolated from the by column chromatography and recrystallization method. The chemical structure of isolate was elucidated by spectroscopic techniques. IC of Tricin and Docetaxel were assessed 117.5 ± 4.4 μM and 0.1 ± 0.02 nM by MTT assay, respectively. Analysis of results indicates the synergistic effect of combination therapy on decreased proliferation. MiR-21 in treated cells with Tricin significantly decreased compared to control cells. So, Tricin can be effective in the reduction of metastasis and drug resistance of Docetaxel.
为了提高疗效并克服化疗药物的耐药性，提出了联合治疗方案。具有不同抗癌作用的黄酮类化合物作为先导化合物是一个合适的选择。前列腺癌中过表达的MiR-21与转移以及对多西他赛化疗的耐药性有关。在本研究中，研究了4',5,7-三羟基-3',5'-二甲氧基黄酮（小麦黄素）与多西他赛对PC3细胞系的抗癌作用。小麦黄素最初通过柱色谱和重结晶法从[此处原文缺失相关信息]中分离得到。通过光谱技术阐明了分离物的化学结构。通过MTT法评估，小麦黄素和多西他赛的IC50分别为117.5±4.4μM和0.1±0.02 nM。结果分析表明联合治疗对降低细胞增殖具有协同作用。与对照细胞相比，用小麦黄素处理的细胞中MiR-21显著降低。因此，小麦黄素可有效降低多西他赛的转移和耐药性。
