Shahrani Mehrdad, Asgharian Shirin, Hosseini Alireza, Bijad Elham, Anjomshoa Maryam, Rostamzadeh Ayoob, Asgharzadeh Najmeh, Lorigooini Zahra
Medical Plants Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran.
Department of Embryology and Histology, Faculty of Medicine, Shahrekord University of Medical Sciences, Shahrekord, Iran.
Biomedicine (Taipei). 2020 Sep 1;10(3):25-32. doi: 10.37796/2211-8039.1026. eCollection 2020.
The most important side effects of Cyclophosphamide, as an anticancer broad-spectrum drug, are the negative effects on the reproduction and fertility because of oxidative stress. Considering the antioxidant properties of medicinal plants, especially those of the genus, this paper studied the effect of hydroalcoholic extract of L. on the pathology of testicular tissue in CP-treated mice.
Groups of this experimental study consisted of normal saline recipients; three groups receiving . extract at 50, 100, 200 mg/kg; three groups receiving extract at 50, 100, and 200 mg/g and 6.6 mg/kg of Cyclophosphamide; and a group given Cyclophosphamide at 1.6 mg/kg. All injections were performed intra-peritoneally. After 30 days, the testicular histological profile as well as the number of spermatozoa, the number of primary and round spermatocytes, and the number of spermatogonia were investigated.
Cyclophosphamide treatment significantly reduced the lumen diameter, the seminiferous tubule diameter, the epithelial thickness, as well as decreased the quantity of spermatozoa and round and primary spermatocytes compared to the control group. Cyclophosphamide groups treated with . extract at 50, 100 and 200 mg/kg in a significant manner improved these variables ( < 0.001).
extract can significantly improve Cyclophosphamide-induced toxicity and pathological process on testicular tissue. It seems that this plant, with high antioxidant capacity, can be considered a complementary therapy for Cyclophosphamide to prevent undesirable effects on the reproductive system.
环磷酰胺作为一种抗癌广谱药物，最重要的副作用是由于氧化应激对生殖和生育能力产生负面影响。考虑到药用植物的抗氧化特性，特别是该属植物的特性，本文研究了L.的水醇提取物对环磷酰胺处理小鼠睾丸组织病理学的影响。
本实验研究的分组包括接受生理盐水的组；三组分别接受50、100、200mg/kg的提取物；三组分别接受50、100、200mg/g的提取物以及6.6mg/kg的环磷酰胺；还有一组给予1.6mg/kg的环磷酰胺。所有注射均通过腹腔内进行。30天后，研究睾丸组织学特征以及精子数量、初级和圆形精母细胞数量以及精原细胞数量。
与对照组相比，环磷酰胺处理显著降低了管腔直径、曲细精管直径、上皮厚度，同时减少了精子数量以及圆形和初级精母细胞数量。用50、100和200mg/kg的提取物处理的环磷酰胺组显著改善了这些变量（<0.001）。
提取物可以显著改善环磷酰胺诱导的睾丸组织毒性和病理过程。看来这种具有高抗氧化能力的植物可以被视为环磷酰胺的一种辅助疗法，以预防对生殖系统的不良影响。
