Tsui Hok Yin, Liu You Chen, Yan Xin, Lin Yue, Xu Ye, Tan Qian
a Department of Burns and Plastic Surgery , Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School , Nanjing , China.
Growth Factors. 2017 Dec;35(6):216-224. doi: 10.1080/08977194.2018.1435532. Epub 2018 Feb 15.
Wounds in patients with diabetes mellitus are one of the most prevalent impaired wounds in the world. Vascular endothelial growth factor (VEGF) is one of the most important proangiogenic mediators. Artificial dermal (AD) such as Pelnac has been shown, in humans and animal models, a great therapeutic potential in full-thickness skin wounds. We attempt to promote the wound healing in diabetic porcine models through combined use of AD and constant concentration of VEGF or VEGF concentration gradient. We created full-thickness excisional wounds in diabetic animal models. Analyzed the healing process through images, histology and immunohistochemistry. Results show that the combination of AD and concentration gradient of VEGF could provide an appropriate angiogenesis, improve granulation formation, increase epithelization and maintain the VEGF levels of the wound bed. Eventually accelerate the direct healing of diabetic wounds or make good preparation for secondary skin graft.
糖尿病患者的伤口是世界上最常见的受损伤口之一。血管内皮生长因子（VEGF）是最重要的促血管生成介质之一。诸如Pelnac之类的人工真皮（AD）在人类和动物模型中已显示出对全层皮肤伤口具有巨大的治疗潜力。我们试图通过联合使用AD和恒定浓度的VEGF或VEGF浓度梯度来促进糖尿病猪模型中的伤口愈合。我们在糖尿病动物模型中制造了全层切除伤口。通过图像、组织学和免疫组织化学分析愈合过程。结果表明，AD与VEGF浓度梯度的组合可以提供适当的血管生成，改善肉芽形成，增加上皮化并维持伤口床的VEGF水平。最终加速糖尿病伤口的直接愈合或为二期皮肤移植做好准备。
