State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China.
The School of Chemistry & Chemical Engineering, State Key Laboratory of Metal Matrix Composites, Shanghai Jiao Tong University, Shanghai 200240, China.
Int J Biol Macromol. 2018 Jul 1;113:983-990. doi: 10.1016/j.ijbiomac.2018.02.062. Epub 2018 Feb 13.
Owing to the ever-increasing environmental and health impacts associated with the discharge of dye-based effluents, effective remediation of industrial waste have become a top priority for the industrialists and environmental fraternity, around the world. Plant-based peroxidases represent a suitable bio-remediating agent for the effective treatment of original dyes or dye-based colored wastewater effluents. In the present study, horseradish peroxidase was immobilized by copolymerization into cross-linked polyacrylamide gel and investigated its potential for the degradation and detoxification of an azo dye, methyl orange. In the presence of N, N'-methylenebisacrylamide as a cross-linker, polyacrylamide gel at 10% concentration furnished >80% of immobilization yield. The surface morphology of the control (free enzyme) and immobilized enzyme, i.e., horseradish peroxidase cross-linked polyacrylamide gel was characterized using scanning electron microscopy. The high yielded horseradish peroxidase cross-linked polyacrylamide gel concentration, i.e., 10% was used to develop a packed bed reactor and exploited for dye degradation and detoxification purposes. A noteworthy level (>90%) of dye degradation was recorded after polyacrylamide gel cross-linked horseradish peroxidase-catalyzed reaction in a packed bed bioreactor. The biodegradation was further assessed by Fourier-transform infrared spectral analysis. The acute toxicity assays demonstrated that enzyme-based bio-degradation might be used effectively for large-scale environmental remediation of dyes and dyes containing wastewater effluents.
由于与排放染料废水相关的环境和健康影响不断增加，因此，全球工业家和环保界人士都将有效处理工业废物作为当务之急。植物过氧化物酶是一种合适的生物修复剂，可有效处理原始染料或基于染料的有色废水。在本研究中，辣根过氧化物酶通过共聚作用固定在交联聚丙烯酰胺凝胶中，并研究了其对偶氮染料甲基橙的降解和解毒潜力。在 N，N'-亚甲基双丙烯酰胺作为交联剂的存在下，10%浓度的聚丙烯酰胺凝胶提供了>80%的固定化产率。使用扫描电子显微镜对对照（游离酶）和固定化酶，即辣根过氧化物酶交联聚丙烯酰胺凝胶的表面形态进行了表征。高产量的辣根过氧化物酶交联聚丙烯酰胺凝胶浓度，即 10%，用于开发填充床反应器，并用于染料降解和解毒目的。在填充床生物反应器中，辣根过氧化物酶催化反应后，记录到染料降解率达到了显著水平（>90%）。通过傅里叶变换红外光谱分析进一步评估了生物降解。急性毒性试验表明，基于酶的生物降解可有效用于大规模环境修复染料和含染料废水。
