Lee Joshua, Kim In Gi, Oh Young Min, Park Chan-Hee, Kim Cheol Sang
Department of Bionanosystem Engineering, Graduate School, Chonbuk National University, Jeonju, Korea.
Department of Neurosurgery, Research Institute of Clinical Medicine, Chonbuk National University Medical School and Hospital, Jeonju, Korea.
J Nanosci Nanotechnol. 2018 Feb 1;18(2):1123-1126. doi: 10.1166/jnn.2018.14862.
We have investigated the effect of flow rate on shear stress and in turn thrombus formation on a lab-on-a-chip with a microchannel that is suitable for cell culture and growth. Using a combination of Arduino UNO, Arduino Motor Shield, and a SERVO stepper motor, we created a pump system that closely mimics the in vivo conditions of the human body. With this system, we achieved continuous flow of blood and observed attached platelets at the bottom of the collagen coated microslide, confirming that with shear stress, thrombus formation increases.
我们研究了流速对剪切应力的影响，进而研究了在适合细胞培养和生长的带有微通道的芯片实验室上血栓形成的情况。通过结合使用Arduino UNO、Arduino电机屏蔽板和一个舵机步进电机，我们创建了一个能紧密模拟人体体内状况的泵系统。利用该系统，我们实现了血液的连续流动，并观察到在胶原涂层微载玻片底部附着的血小板，证实了随着剪切应力的增加，血栓形成也会增加。
