Kaarj Kattika, Madias Marianne, Akarapipad Patarajarin, Cho Soohee, Yoon Jeong-Yeol
Department of Biosystems Engineering, The University of Arizona, Tucson, AZ USA.
Department of Biomedical Engineering, The University of Arizona, Tucson, AZ USA.
J Biol Eng. 2020 Jul 28;14:20. doi: 10.1186/s13036-020-00242-5. eCollection 2020.
Mechanical stimuli play important roles on the growth, development, and behavior of tissue. A simple and novel paper-based in vitro tissue chip was developed that can deliver two types of mechanical stimuli-local compression and shear flow-in a programmed manner. Rat vascular endothelial cells (RVECs) were patterned on collagen-coated nitrocellulose paper to create a tissue chip. Localized compression and shear flow were introduced by simply tapping and bending the paper chip in a programmed manner, utilizing an inexpensive servo motor controlled by an Arduino microcontroller and powered by batteries. All electrical compartments and a paper-based tissue chip were enclosed in a single 3D-printed enclosure, allowing the whole device to be independently placed within an incubator. This simple device effectively simulated in vivo conditions and induced successful RVEC migration in as early as 5 h. The developed device provides an inexpensive and flexible alternative for delivering mechanical stimuli to other in vitro tissue models.
机械刺激对组织的生长、发育和行为起着重要作用。我们开发了一种简单新颖的基于纸张的体外组织芯片，它能够以编程方式施加两种类型的机械刺激——局部压缩和剪切流。将大鼠血管内皮细胞（RVECs）接种在胶原包被的硝酸纤维素纸上，制成组织芯片。利用由Arduino微控制器控制并由电池供电的廉价伺服电机，通过以编程方式简单敲击和弯曲纸片来引入局部压缩和剪切流。所有电气部件和基于纸张的组织芯片都封装在一个3D打印的外壳中，使整个装置能够独立放置在培养箱内。这种简单的装置有效地模拟了体内条件，并早在5小时内就成功诱导了RVEC迁移。所开发的装置为向其他体外组织模型施加机械刺激提供了一种廉价且灵活的替代方案。
