Institute of Medical Biotechnology, Friedrich-Alexander-University Erlangen-Nürnberg, 91052 Erlangen, Bavaria, Germany.
Institute of Medical Biotechnology, Friedrich-Alexander-University Erlangen-Nürnberg, 91052 Erlangen, Bavaria, Germany.
Vaccine. 2018 Mar 14;36(12):1545-1547. doi: 10.1016/j.vaccine.2018.01.080. Epub 2018 Feb 12.
The human complement system is the most effective defense mechanism of the human innate immune system. One major negative regulator of the alternative pathway in human blood is complement factor H (FH). It binds to autologous cells and thus, prevents complement attack against body-cells or tissues. Various pathogens are known to escape complement recognition by recruiting FH to provide protection against the host's immune system. This immune evasion mechanism was recently qualitatively reported for asexual malaria blood stages. To indirectly evaluate the stage-specific potential of FH-receptor proteins as vaccine candidates, we quantified the FH molecules bound to the surface of different malaria blood stage parasites by Western blot and a commercially available FH-ELISA, which was originally designed to measure the FH concentration in human serum. Host-cell-free merozoites and intracellular mature schizont (here called segmenter) stages bind significantly more FH molecules than earlier parasite stages.
人体补体系统是人体先天免疫系统中最有效的防御机制。在人体血液中，替代途径的一个主要负调控因子是补体因子 H（FH）。它与自身细胞结合，从而防止补体攻击自身细胞或组织。已知各种病原体通过招募 FH 来逃避补体识别，从而提供对宿主免疫系统的保护。这种免疫逃避机制最近在无性疟原虫血阶段被定性报道。为了间接评估 FH 受体蛋白作为疫苗候选物的阶段特异性潜力，我们通过 Western blot 和一种商业上可用的 FH-ELISA 来定量测定不同疟原虫血阶段寄生虫表面结合的 FH 分子，该 ELISA 最初旨在测量人血清中的 FH 浓度。无宿主细胞的裂殖子和细胞内成熟裂殖体（这里称为分节体）阶段比早期寄生虫阶段结合更多的 FH 分子。
