Pelletier L, Pasquier R, Hirsch F, Sapin C, Druet P
J Immunol. 1986 Oct 15;137(8):2548-54.
Mercuric chloride induces in Brown-Norway rats an autoimmune disease due to a T dependent polyclonal activation of B cells. Various autoantibodies and a striking increase in total serum IgE level are observed as consequences of this polyclonal activation. The aim of this study was to investigate the in vitro response of autologous syngeneic normal lymphocytes to lymphocytes exposed in vivo or in vitro to HgCL2. Helper/inducer T cells (W3/25 +) exposed to HgCl2 were found to stimulate normal T lymphocytes in the presence of normal Ia (+) cells. The proliferating T cells also had the helper/inducer phenotype. To demonstrate the potential relevance of this in vitro phenomenon to the autoimmune disease, HgCl2-pretreated T cells were injected into the footpads of normal syngeneic recipients. Draining popliteal lymph nodes contained a highly significant number of both surface IgE positive and IgE containing cells. These experiments demonstrate that HgCl2 induces autoreactive T cells and suggest that these cells may be responsible for the autoimmune disease.
氯化汞可在棕色挪威大鼠中诱发一种自身免疫性疾病，这是由于B细胞的T细胞依赖性多克隆激活所致。作为这种多克隆激活的结果，可观察到各种自身抗体以及血清总IgE水平显著升高。本研究的目的是调查自体同基因正常淋巴细胞对体内或体外暴露于HgCL2的淋巴细胞的体外反应。发现暴露于HgCl2的辅助/诱导性T细胞（W3/25 +）在正常Ia（+）细胞存在的情况下能够刺激正常T淋巴细胞。增殖的T细胞也具有辅助/诱导性表型。为了证明这种体外现象与自身免疫性疾病的潜在相关性，将经HgCl2预处理的T细胞注射到正常同基因受体的足垫中。引流的腘窝淋巴结中含有大量表面IgE阳性和含IgE的细胞。这些实验表明，HgCl2可诱导自身反应性T细胞，并提示这些细胞可能是自身免疫性疾病的病因。
