Okimura T, Nagata I
J Immunopharmacol. 1986;8(3):327-46. doi: 10.3109/08923978609026493.
Oral administration of diazepam at doses of 5-10 mg/kg to restraint-stressed mice resulted in almost complete recovery in the stress-induced suppression of the antibody response to sheep red blood cell (SRBC). Moreover, this compound restored the suppression of antibody response to SRBC in cyclophosphamide-treated mice. Diazepam treatment also enhanced the antibody response against SRBC in normal mice only when the animals were immunized with the reduced amount of antigen. It was demonstrated that antigen specific helper T cell activity was promoted by diazepam administration in mice. Addition of diazepam augmented the in vitro anti-SRBC hemolytic plaque-forming cell (PFC) response in mouse splenocytes without altering kinetics of the response. However, the enhancing effect was observed only when the drug was added to the medium at the culture initiation. On the other hand, antibody response to T cell-independent antigens such as trinitrophenylated (TNP)-Ficoll and TNP-lipopolysaccharide were not enhanced by diazepam. Concanavalin A or LPS-induced 3H-thymidine uptake into splenocytes were not stimulated by diazepam. These results suggest that diazepam promotes the antibody response through stimulating helper T cell functions.
给受束缚应激的小鼠口服5-10毫克/千克剂量的地西泮，可使应激诱导的对绵羊红细胞（SRBC）抗体反应的抑制几乎完全恢复。此外，该化合物还恢复了环磷酰胺处理小鼠中对SRBC抗体反应的抑制。仅当用减少量的抗原免疫动物时，地西泮处理才增强正常小鼠中针对SRBC的抗体反应。已证明，给小鼠施用安定可促进抗原特异性辅助性T细胞活性。添加地西泮可增强小鼠脾细胞体外抗SRBC溶血空斑形成细胞（PFC）反应，而不改变反应动力学。然而，仅当在培养开始时将药物添加到培养基中时才观察到增强作用。另一方面，地西泮不会增强对三硝基苯基化（TNP）-Ficoll和TNP-脂多糖等非T细胞依赖性抗原的抗体反应。地西泮不会刺激伴刀豆球蛋白A或LPS诱导的脾细胞摄取3H-胸腺嘧啶核苷。这些结果表明，地西泮通过刺激辅助性T细胞功能来促进抗体反应。
