Mudzinski S P, Rudofsky U H, Mitchell D G, Lawrence D A
Toxicol Appl Pharmacol. 1986 Apr;83(2):321-30. doi: 10.1016/0041-008x(86)90309-1.
The effect of administration of lead acetate (10 mM in the drinking water) for 8 weeks on the in vivo sheep red blood cell (SRBC) specific plaque-forming cell (PFC) responses of inbred A, BALB/c, C57Bl/6, DBA/1, SJL, and NZW/NZB F1 mice and outbred CFW mice was examined to determine if lead was immunomodulatory in a genetically related manner. Lead did not suppress the SRBC-specific PFC/10(6) splenocytes or PFC/spleen response in any mouse strain when compared to the responses of strain-matched control mice. In addition, 10 mM lead-treated BALB/c mice manifested augmented PFC/10(6) splenocytes (17%; p less than .05) but unchanged PFC/spleen responses. Correspondingly, serum concentrations of SRBC-specific antibody (measured by radioimmunoassay) and serum immunoglobulin G, M, or A isotypes were also unchanged by lead acetate treatment in all tested mouse strains. There were no observable lead-related histopathological changes or deposition of immune complexes or antibasement membrane antibody in the kidneys of treated mice. Further, splenocytes from lead-treated, SRBC-immunized mice cultured with T-independent antigens (TNP-LPS, TNP-Ficoll) or with a T-dependent antigen (SRBC) exhibited direct and indirect specific PFC responses that were unchanged from those of control mice. The H-2K/D haplotypes of the outbred CFW mice were determined by microcytotoxicity to include r, q, u, and s. These results suggest that lead acetate (10 mM) administered po for 8 weeks does not suppress the primary direct humoral immune response to SRBC in inbred and outbred mice of several H-2 haplotypes (k/d; d; b; q; d,z; s; r; and u).
研究了给予醋酸铅（饮用水中浓度为10 mM）8周对近交系A、BALB/c、C57Bl/6、DBA/1、SJL和NZW/NZB F1小鼠以及远交系CFW小鼠体内绵羊红细胞（SRBC）特异性空斑形成细胞（PFC）反应的影响，以确定铅是否以基因相关的方式具有免疫调节作用。与品系匹配的对照小鼠的反应相比，铅在任何小鼠品系中均未抑制SRBC特异性PFC/10⁶脾细胞或PFC/脾脏反应。此外，经10 mM铅处理的BALB/c小鼠表现出PFC/10⁶脾细胞增加（17%；p小于0.05），但PFC/脾脏反应未改变。相应地，在所有测试的小鼠品系中，醋酸铅处理也未改变SRBC特异性抗体（通过放射免疫测定）的血清浓度以及血清免疫球蛋白G、M或A同种型。在处理过的小鼠肾脏中未观察到与铅相关的组织病理学变化、免疫复合物沉积或抗基底膜抗体。此外，用非T依赖性抗原（TNP-LPS、TNP-Ficoll）或T依赖性抗原（SRBC）培养的经铅处理、SRBC免疫的小鼠的脾细胞表现出的直接和间接特异性PFC反应与对照小鼠的反应无变化。通过微量细胞毒性法确定远交系CFW小鼠的H-2K/D单倍型包括r、q、u和s。这些结果表明，经口给予醋酸铅（10 mM）8周不会抑制几种H-2单倍型（k/d；d；b；q；d,z；s；r；和u）的近交和远交小鼠对SRBC的初次直接体液免疫反应。
