Hartter E, Woloszczuk W
J Clin Chem Clin Biochem. 1986 Aug;24(8):559-63. doi: 10.1515/cclm.1986.24.8.559.
Human atrial natriuretic peptide and arginine vasopressin were coextracted from EDTA-plasma with Sep-Pak C18 cartridges, and the hormones were coeluted from the cartridges by a solution containing 900 ml/l of methanol, 5 ml/l of trifluoroacetic acid and 95 ml/l of water. After volume reduction under nitrogen the eluates were lyophilized, dissolved in buffer and aliquots were used for radioimmunological determination of both hormones. Both procedures used delayed addition of [125I]-labelled tracer to enhance sensitivity. Recoveries were 95 +/- 2% for human atrial natriuretic peptide and 96 +/- 3% vor arginine vasopressin (mean +/- SD). Minimal detectable doses were 2 pg/tube of human atrial natriuretic peptide and 0.5 pg/tube of arginine vasopressin (Bo - 3 SD). Intra-assay variabilities were +/- 8% for both tests. Simultaneous extraction of both hormones with high recovery was achieved, thus increasing sensitivity and specificity, at the same time reducing sample volume requirements and technician time.
用人心房利钠肽和精氨酸加压素从乙二胺四乙酸（EDTA）血浆中通过Sep-Pak C18柱共同提取，然后用含有900毫升/升甲醇、5毫升/升三氟乙酸和95毫升/升水的溶液将这些激素从柱上共同洗脱下来。在氮气下进行体积缩减后，洗脱液进行冻干，溶解于缓冲液中，取等分试样用于两种激素的放射免疫测定。两种方法均采用延迟添加[125I]标记的示踪剂以提高灵敏度。人心房利钠肽的回收率为95±2%，精氨酸加压素的回收率为96±3%（平均值±标准差）。人心房利钠肽的最低可检测剂量为2皮克/管，精氨酸加压素的最低可检测剂量为0.5皮克/管（Bo - 3标准差）。两种检测的批内变异率均为±8%。实现了两种激素的同时高回收率提取，从而提高了灵敏度和特异性，同时减少了样品体积需求和技术人员的操作时间。
