Penney M D, Hampton D, Oleesky D A, Livingstone C, Mulkerrin E
Department of Chemical Pathology, Royal Gwent Hospital, Newport, UK.
Ann Clin Biochem. 1992 Nov;29 ( Pt 6):652-8. doi: 10.1177/000456329202900606.
A rapid vacuum-driven procedure, using pre-treated Sep-Pak C18 cartridges, has been developed for the simultaneous extraction of arginine vasopressin (AVP) and atrial natriuretic peptide (ANP) from plasma. Non-specific interference was removed by fractional elution with an aqueous methanol/trifluoroacetic acid (TFA) mixture. AVP and ANP were coeluted under positive pressure with a methanol/TFA mixture and the eluates air-dried before measurement using separate radioimmunoassays. Assay ranges for AVP and ANP were 0.12-29.5 pmol/L and 0.65-162 pmol/L, respectively, with mean recoveries (standard deviation in parentheses) for AVP of 96.4% (5.5%) at a level of 11.8 pmol/L and for ANP of 94.8% (5.9%) at a level of 32.4 pmol/L. The extraction and assay procedures were validated by observing the changes in plasma AVP and ANP concentrations in normal subjects at different stages of hydration and in elderly patients during treatment for congestive cardiac failure.
已开发出一种快速真空驱动程序，使用预处理的Sep-Pak C18柱，用于从血浆中同时提取精氨酸加压素（AVP）和心房利钠肽（ANP）。通过用甲醇/三氟乙酸（TFA）水溶液混合物进行分步洗脱去除非特异性干扰。AVP和ANP在正压下用甲醇/TFA混合物共洗脱，洗脱液在使用单独的放射免疫测定法测量前进行空气干燥。AVP和ANP的测定范围分别为0.12 - 29.5 pmol/L和0.65 - 162 pmol/L，在11.8 pmol/L水平时AVP的平均回收率（括号内为标准差）为96.4%（5.5%），在32.4 pmol/L水平时ANP的平均回收率为94.8%（5.9%）。通过观察正常受试者在不同水化阶段以及老年充血性心力衰竭患者治疗期间血浆AVP和ANP浓度的变化，对提取和测定程序进行了验证。
