Ge Fei, Zhu Longbao, Aang Anna, Song Ping, Li Wanzhen, Tao Yugui, Du Guocheng
School of Biochemical Engineering, Anhui Polytechnic University, 8 Zheshan Road, Wuhu, 241000, Anhui, People's Republic of China.
Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, 214122, People's Republic of China.
Biotechnol Lett. 2018 May;40(5):847-854. doi: 10.1007/s10529-018-2526-3. Epub 2018 Feb 15.
Yeast has been increasingly used as a host for the expression of enzymes. Compared to other expression systems, the yeast expression system has many advantages including its suitability for large-scale fermentation and its ability to modify enzymes. When expressed in yeast, many recombinant enzymes are N-glycosylated, and this may play an important role in their activity, thermostability and secretion. Although the mechanism underlying this process is not clear, the regulation of N-glycosylation by introducing or eliminating N-glycosylation at specific sites has developed into an important strategy for improving the production or catalytic properties of recombinant enzymes. In this review, we summarize the recent advances in understanding the effects of N-glycosylation on the expression and characteristics of recombinant enzymes, and discuss novel strategies for regulating N-glycosylation in yeast. We hope that this review will help improve the understanding of the expression and the catalytic properties of N-glycosylated proteins.
酵母已越来越多地被用作酶表达的宿主。与其他表达系统相比，酵母表达系统具有许多优点，包括适合大规模发酵以及对酶进行修饰的能力。当在酵母中表达时，许多重组酶会进行N-糖基化，这可能在其活性、热稳定性和分泌中发挥重要作用。尽管这一过程的潜在机制尚不清楚，但通过在特定位点引入或消除N-糖基化来调控N-糖基化已发展成为提高重组酶产量或催化特性的重要策略。在这篇综述中，我们总结了在理解N-糖基化对重组酶表达和特性影响方面的最新进展，并讨论了在酵母中调控N-糖基化的新策略。我们希望这篇综述将有助于增进对N-糖基化蛋白表达和催化特性的理解。
