[用补骨脂素复性和交联的DNA片段的电泳：DNA光交联的动力学分析]
[Electrophoresis of DNA fragments renatured and cross-linked with psoralen: kinetic analysis of DNA photocross-linking].
作者信息
Gonikberg E M, Andreev V M
出版信息
Mol Biol (Mosk). 1986 Jul-Aug;20(4):1039-47.
PMID:2945091
Abstract
The kinetics of photoreactions between DNA and psoralen was analysed. A method is described for determining the dependence of the average number of cross-links per base-pair (n) on the fluence of UV-irradiation. This calibration curve allows to specify the conditions of irradiation necessary to obtain any desired value of n.
摘要
分析了DNA与补骨脂素之间光反应的动力学。描述了一种用于确定每个碱基对交联平均数（n）对紫外线照射通量依赖性的方法。该校准曲线允许指定获得任何所需n值所需的照射条件。
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