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[用补骨脂素复性和交联的DNA片段的电泳:DNA光交联的动力学分析]

[Electrophoresis of DNA fragments renatured and cross-linked with psoralen: kinetic analysis of DNA photocross-linking].

作者信息

Gonikberg E M, Andreev V M

出版信息

Mol Biol (Mosk). 1986 Jul-Aug;20(4):1039-47.

PMID:2945091
Abstract

The kinetics of photoreactions between DNA and psoralen was analysed. A method is described for determining the dependence of the average number of cross-links per base-pair (n) on the fluence of UV-irradiation. This calibration curve allows to specify the conditions of irradiation necessary to obtain any desired value of n.

摘要

分析了DNA与补骨脂素之间光反应的动力学。描述了一种用于确定每个碱基对交联平均数(n)对紫外线照射通量依赖性的方法。该校准曲线允许指定获得任何所需n值所需的照射条件。

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[Electrophoresis of DNA fragments renatured and cross-linked with psoralen: kinetic analysis of DNA photocross-linking].[用补骨脂素复性和交联的DNA片段的电泳:DNA光交联的动力学分析]
Mol Biol (Mosk). 1986 Jul-Aug;20(4):1039-47.
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[Measuring the bleomycin-induced paired damage of two DNA chains by a method of psoralen photocross-linking].[通过补骨脂素光交联法测量博来霉素诱导的两条DNA链的配对损伤]
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Mechanism of site-specific psoralen photoadducts formation in triplex DNA directed by psoralen-conjugated oligonucleotides.由补骨脂素缀合寡核苷酸引导的三链 DNA 中位点特异性补骨脂素光加合物形成的机制。
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[Use of psoralen for detecting the conjugated damage to 2 chains in irradiated phage PM2 DNA].
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