Gonikberg E M, Odintsova S P
Mol Biol (Mosk). 1986 Jul-Aug;20(4):1048-52.
DNA of bacteriophage PM2 was allowed to react with bleomycin in the presence of Fe(II) and oxygen and the "paired" DNA lesions of two types were measured: (1) double-strand breaks, (2) lesions converted to double-strand breaks after introducing into the DNA a large number of psoralen cross-links (about 10(-2) per base pair) and alkali treatment. The mean numbers of each lesion type per DNA molecule are found to be proportional to the square of bleomycin concentration over the range of 3 X 10(-7) to 3 X 10(-6) M. These findings indicate that paired lesions are formed as a result of action of two bleomycin molecules at the same DNA site.
在亚铁离子（Fe(II)）和氧气存在的条件下，使噬菌体PM2的DNA与博来霉素发生反应，并测定了两种类型的“配对”DNA损伤：（1）双链断裂；（2）在DNA中引入大量补骨脂素交联（约每碱基对10^(-2)个）并经过碱处理后转化为双链断裂的损伤。发现在3×10^(-7)至3×10^(-6)M的浓度范围内，每个DNA分子中每种损伤类型的平均数量与博来霉素浓度的平方成正比。这些发现表明，配对损伤是由两个博来霉素分子作用于同一DNA位点而形成的。
