Department of Chemistry, Massachusetts Institute of Technology, USA.
Nanoscale. 2018 Mar 1;10(9):4406-4414. doi: 10.1039/c7nr08673c.
We have developed a strategy for synthesizing immediately activable, water-soluble, compact (∼10-12 nm hydrodynamic diameter) quantum dots with a small number of stable and controllable conjugation handles for long distance delivery and subsequent biomolecule conjugation. Upon covalent conjugation with engineered monovalent streptavidin, the sample results in a population consisting of low-valency quantum dots. Alternatively, we have synthesized quantum dots with a small number of biotin molecules that can self-assemble with engineered divalent streptavidin via high-affinity biotin-streptavidin interactions. Being compact, stable and highly specific against biotinylated proteins of interest, these low-valency quantum dots are ideal for labeling and tracking single molecules on the cell surface with high spatiotemporal resolution for different biological systems and applications.
我们开发了一种合成策略，用于制备立即可激活、水溶性、紧凑（~10-12nm 水动力直径）的量子点，这些量子点具有少量稳定可控的连接臂，可用于长距离输送和随后的生物分子偶联。通过与工程化单价链霉亲和素共价偶联，样品得到了由低价量子点组成的群体。或者，我们已经合成了具有少量生物素分子的量子点，这些量子点可以通过高亲和力的生物素-链霉亲和素相互作用与工程化的二价链霉亲和素自组装。这些低价量子点由于其紧凑、稳定和对感兴趣的生物素化蛋白具有高度特异性，非常适合用于不同生物系统和应用中以高时空分辨率标记和跟踪细胞表面上的单个分子。
