Lieberman J, Verret C R, Kranz D M, Hubbard S C, Saito H, Raulet D H, Tonegawa S, Eisen H N
Proc Natl Acad Sci U S A. 1986 Oct;83(20):7870-4. doi: 10.1073/pnas.83.20.7870.
The previously determined sequence of the murine T-cell gamma gene and its transcription in cloned T lymphocytes suggests that the polypeptide encoded by this gene is generally present in cytotoxic T cells as a 33-kDa monomer in a disulfide-bonded dimer. The gamma chain is also expected to be phosphorylated because a sequence in its cytoplasmic domain is homologous to an active site for serine phosphorylation in the regulatory subunit of cAMP-dependent protein kinase. We describe here a cytotoxic-T-cell-associated phosphorylated protein, many of whose properties suggest that it may be the product of the T-cell gamma gene. Its phosphorylation is greatly enhanced by interleukin 2 stimulation.
先前确定的小鼠T细胞γ基因序列及其在克隆的T淋巴细胞中的转录表明，该基因编码的多肽通常以二硫键结合的二聚体形式作为33 kDa单体存在于细胞毒性T细胞中。γ链也有望被磷酸化，因为其胞质结构域中的一个序列与环磷酸腺苷依赖性蛋白激酶调节亚基中丝氨酸磷酸化的活性位点同源。我们在此描述一种与细胞毒性T细胞相关的磷酸化蛋白，其许多特性表明它可能是T细胞γ基因的产物。白细胞介素2刺激可大大增强其磷酸化。
