Stuhler G, Walden P
Max-Planck-Institute for Biology, Tubingen, FRG.
Eur J Immunol. 1993 Sep;23(9):2279-86. doi: 10.1002/eji.1830230934.
Cytotoxic T lymphocytes are major effector cells in response to viral infections and in allograft rejection and are implicated in many other immunological reactions. Efficient induction of cytotoxic activity in these cells in many but not all cases depends upon helper T and antigen-presenting cells so that at least three different cell types have to work together. Here we present an in vitro model for the helper T cell-dependent induction of cytotoxic T lymphocytes which allows the investigation of the collaboration of helper and cytotoxic T cells. First results demonstrate that linkage of helper and killer epitopes on the surface of one antigen-presenting cell is a prerequisite for productive interaction between the two T cells that results in induction of cytolytic activity. T helper 1 and T helper 2 cells are equally efficient. The crucial roles of interleukin-2 and interferon-gamma in this process were confirmed. Activated CD4 cells can influence cytotoxic T lymphocytes in such a way that they produce interferon-gamma independent from recognition of cognate peptide. The possibility of direct T-T contacts mediated by adhesion molecules that promote collaboration of the two cells is discussed.
细胞毒性T淋巴细胞是应对病毒感染和同种异体移植排斥反应的主要效应细胞，并参与许多其他免疫反应。在许多但并非所有情况下，这些细胞中细胞毒性活性的有效诱导依赖于辅助性T细胞和抗原呈递细胞，因此至少三种不同的细胞类型必须协同工作。在此，我们展示了一种用于辅助性T细胞依赖性诱导细胞毒性T淋巴细胞的体外模型，该模型允许研究辅助性T细胞和细胞毒性T细胞之间的协作。初步结果表明，在一个抗原呈递细胞表面上辅助性表位和杀伤性表位的连接是导致细胞溶解活性诱导的两个T细胞之间有效相互作用的先决条件。辅助性T1细胞和辅助性T2细胞同样有效。白细胞介素-2和干扰素-γ在这一过程中的关键作用得到了证实。活化的CD4细胞可以以这样一种方式影响细胞毒性T淋巴细胞，即它们产生独立于同源肽识别的干扰素-γ。讨论了由促进两种细胞协作的黏附分子介导的直接T-T接触的可能性。
