State Key Laboratory of Agro-Biotechnology and Key Laboratory of Pest Monitoring and Green Management-MOA, China Agricultural University, No.2 Yuanmingyuan West Road, Beijing 100193, China; College of Plant Protection, Shenyang Agricultural University, No.120 Dongling Road, Shenyang 110866, China.
State Key Laboratory of Agro-Biotechnology and Key Laboratory of Pest Monitoring and Green Management-MOA, China Agricultural University, No.2 Yuanmingyuan West Road, Beijing 100193, China.
Plant Physiol Biochem. 2018 Apr;125:143-152. doi: 10.1016/j.plaphy.2018.01.031. Epub 2018 Jan 31.
MicroRNAs (miRNAs) are endogenous non-coding small RNAs that play essential regulatory roles in plant development and environmental stress responses. Maize (Zea mays L.) is a global economically important food and forage crop. To date, a number of maize miRNAs have been identified as being involved in plant development and stress responses. However, the miRNA-mediated gene regulatory networks responsive to virus infections in maize remain largely unknown. In this study, the profiles of small RNAs in buffer- and Sugarcane mosaic virus (SCMV)-inoculated maize plants were obtained by high-throughput sequencing, respectively. A total of 154 known miRNAs and 213 novel miRNAs were profiled and most of the miRNAs identified were differentially expressed after SCMV infection. In addition, 70 targets of 13 known miRNAs and 3 targets of a novel miRNA were identified by degradome analysis. The results of Northern blotting and quantitative real-time PCR showed that the expression levels of the selected miRNAs and their targets were mostly influenced by SCMV infection at 12 days post inoculation, including up-regulation of miR168 and miR528, and down-regulation of miR159, miR397 and miR827. These results provide new insights into the regulatory networks of miRNAs and their targets in maize plants responsive to SCMV infection.
微小 RNA（miRNA）是内源性非编码小分子 RNA，在植物发育和环境胁迫响应中发挥重要的调节作用。玉米（Zea mays L.）是全球重要的经济粮食和饲料作物。迄今为止，已经发现许多玉米 miRNA 参与植物发育和应激反应。然而，miRNA 介导的对病毒感染响应的基因调控网络在玉米中仍很大程度上未知。在这项研究中，通过高通量测序分别获得了缓冲液和甘蔗花叶病毒（SCMV）接种玉米植株的小 RNA 图谱。共鉴定出 154 个已知 miRNA 和 213 个新的 miRNA，大多数鉴定出的 miRNA 在 SCMV 感染后表达差异。此外，通过降解组分析鉴定了 13 个已知 miRNA 的 70 个靶标和 1 个新 miRNA 的 3 个靶标。Northern 印迹和定量实时 PCR 的结果表明，在接种后 12 天，选择的 miRNA 及其靶标受 SCMV 感染的影响最大，包括 miR168 和 miR528 的上调，以及 miR159、miR397 和 miR827 的下调。这些结果为玉米植物对 SCMV 感染响应的 miRNA 和其靶标调控网络提供了新的见解。
