Ghorbani Abozar, Izadpanah Keramatollah, Tahmasebi Ahmad, Afsharifar Alireza, Moghadam Ali, Dietzgen Ralf G
Plant Virology Research Center, College of Agriculture, Shiraz University, Shiraz, Iran.
Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, St. Lucia, QLD Australia.
3 Biotech. 2022 Mar;12(3):69. doi: 10.1007/s13205-022-03134-1. Epub 2022 Feb 12.
MicroRNAs (miRNAs) play key regulatory roles in the plant's response to biotic and abiotic stresses and have fundamental functions in plant-virus interactions. The study of changes in miRNAs in response to virus infection can provide molecular details for a better understanding of virus-host interactions. Maize Iranian mosaic virus (MIMV) infects maize and certain other poaceous plants but miRNA changes in response to MIMV infection are unknown. In the present study, we compared the miRNA profiles of MIMV-infected and uninfected maize and characterized their predicted roles in response to the virus. Small RNA sequencing of maize identified 257 conserved miRNAs of 26 conserved families in uninfected and MIMV-infected maize libraries. Among them, miR395, miR166 and miR156 family members were highly represented. Small RNA data were confirmed using RT-qPCR. In addition, 33 potential novel miRNAs were predicted. The data show that 13 miRNAs were up-regulated and 113 were down-regulated in response to MIMV infection. Several of those miRNAs are known to be important in the response to plant pathogens. To determine the potential roles of individual miRNAs in response to MIMV, miRNA targets, predicted interactions with circular RNAs and comparative transcriptome data were analyzed. The expression profiles of different miRNAs in response to MIMV provide novel insights into the roles of miRNAs in the interaction between MIMV and maize plants.
The online version contains supplementary material available at 10.1007/s13205-022-03134-1.
微小RNA（miRNA）在植物对生物和非生物胁迫的响应中发挥关键调节作用，并且在植物与病毒的相互作用中具有基本功能。研究miRNA响应病毒感染的变化可为更好地理解病毒与宿主的相互作用提供分子细节。伊朗玉米花叶病毒（MIMV）感染玉米和某些其他禾本科植物，但miRNA响应MIMV感染的变化尚不清楚。在本研究中，我们比较了感染MIMV和未感染MIMV的玉米的miRNA谱，并确定了它们在应对病毒时的预测作用。对玉米进行小RNA测序，在未感染和感染MIMV的玉米文库中鉴定出26个保守家族的257个保守miRNA。其中，miR395、miR166和miR156家族成员占比很高。小RNA数据通过RT-qPCR得到证实。此外，预测了33个潜在的新miRNA。数据表明，13个miRNA在响应MIMV感染时上调，113个下调。其中一些miRNA已知在植物病原体响应中很重要。为了确定单个miRNA在响应MIMV时的潜在作用，分析了miRNA靶标、与环状RNA的预测相互作用以及比较转录组数据。不同miRNA响应MIMV的表达谱为miRNA在MIMV与玉米植株相互作用中的作用提供了新的见解。
在线版本包含可在10.1007/s13205-022-03134-1获取的补充材料。
