支持细胞的吞噬作用：通过共聚焦显微镜和电子显微镜对主要吞噬步骤的分析
Phagocytosis by Sertoli Cells: Analysis of Main Phagocytosis Steps by Confocal and Electron Microscopy.
作者信息
Yefimova Marina G, Messaddeq Nadia, Meunier Annie-Claire, Cantereau Anne, Jegou Bernard, Bourmeyster Nicolas
机构信息
Laboratoire Signalisation et Transports Ioniques Membranaires, Université de Poitiers/CNRS, Poitiers, France.
Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences, St. Petersbourg, Russia.
出版信息
Methods Mol Biol. 2018;1748:85-101. doi: 10.1007/978-1-4939-7698-0_8.
Sertoli cells were discovered in the seminiferous tubules by Enrico Sertoli in 1865 (Morgagni 7:31-33, 1865). Intense phagocytosis is, in the context of spermatogenesis cycle, morphologically the most noticeable function of Sertoli cells. In this chapter the major principles of phagocytosis machinery and its specificities in the seminiferous tubules will be briefly reviewed, guidelines of analysis of main phagocytosis steps by confocal and transmission electron microscopy will be described, and a simplified method to assess phagocytosis rate in routine experiments will be given.
1865年，恩里科·塞尔托利在生精小管中发现了塞尔托利细胞（莫尔加尼7:31 - 33，1865年）。在精子发生周期的背景下，强烈的吞噬作用在形态学上是塞尔托利细胞最显著的功能。在本章中，将简要回顾吞噬机制的主要原理及其在生精小管中的特异性，描述通过共聚焦显微镜和透射电子显微镜分析主要吞噬步骤的指南，并给出一种在常规实验中评估吞噬率的简化方法。
Zotero 插件